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Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption
Niu, Jian-Feng1; Chen, Zhang-Fan1; Wang, Guang-Ce1,2; Zhou, Bai-Cheng1
2010-02-01
发表期刊JOURNAL OF APPLIED PHYCOLOGY
卷号22期号:1页码:25-31
文章类型Article
摘要R-phycoerythrin was purified by means of phenyl-sepharose expanded bed absorption and DEAE-sepharose ion-exchange chromatography from Porphyra yezoensis, one of the largest and important aquaculture species in China. Final R-phycoerythrin preparation was characterized by purity ratio above 4 and the homogeneity in native PAGE, respectively. The results of absorption spectrum, fluorescence spectrum and SDS-PAGE were in agreement with previous reports on R-PE. The yield of R-phycoerythrin was 0.82 mg g(-1) wet leafy gametophyte of P. yezoensis. This method is a high-protein recovery technology while reducing processing time, and is suitable for the large batch production of R-phycoerythrin, which will enhance the value of P. yezoensis in China, especially the inferior P. yezoensis which can not be used for flake processing.
关键词Porphyra Yezoensis R-phycoerythrin Hydrophobic Interaction Chromatography Expanded Bed Absorption Ion-exchange Chromatography Streamline Column
DOI10.1007/s10811-009-9420-2
收录类别SCI
语种英语
WOS记录号WOS:000274435300003
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32844
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China
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GB/T 7714
Niu, Jian-Feng,Chen, Zhang-Fan,Wang, Guang-Ce,et al. Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption[J]. JOURNAL OF APPLIED PHYCOLOGY,2010,22(1):25-31.
APA Niu, Jian-Feng,Chen, Zhang-Fan,Wang, Guang-Ce,&Zhou, Bai-Cheng.(2010).Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption.JOURNAL OF APPLIED PHYCOLOGY,22(1),25-31.
MLA Niu, Jian-Feng,et al."Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption".JOURNAL OF APPLIED PHYCOLOGY 22.1(2010):25-31.
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