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Molecular cloning and expression of NOS in shrimp, Litopenaeus vannamei
Yao, Cui-Luan2; Ji, Pei-Feng2; Wang, Zhi-Yong2; Li, Fu-hua1; Xiang, Jian-Hai1
2010-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume28Issue:3Pages:453-460
SubtypeArticle
AbstractThe importance of the nitric oxide synthase (NOS) gene family is demonstrated by many studies in recent years However, the lack of sequence information and clones of shrimp NOS cDNA limits further study on its characterization and function in this species In this report, the cDNA of NOS contained full-length ORF was cloned from the Pacific white shrimp, Litopenaeus vannamei It was of 4680 bp, including a 5'-terminal untranslated region (UTR) of 278 bp, a 3'-terminal UTR of 862 bp, which contained 5 ATTTA repeats, and an open reading frame (ORF) of 3540 bp encoding a polypeptide of 1179 amino acids It contained a typical NO synthase domain at the N-terminal, next to a flavodoxin I domain, a flavin adenine dinucleotide (FAD) binding domain, respectively, and a conservative nicotinamide adenine dinucleotide (NAD) binding domain structure at the C-terminal Quantitative real-time reverse transcription PCR analysis revealed L vannamei NOS (LvNOS) to be expressed in most shrimp tissues, with highest expression in the hepatopancreas and weakest expression in skin. The expression of LvNOS after challenge with LPS and poly I:C was tested in hemocytes, hepatopancreas and nerve The results indicated that the NOS transcript level could be induced in hemocytes by injection with LPS The highest expression was in the hemocyte. with 8 8 times (at 3 h) as much as that in the control (p < 0.05) However, sharp clown-regulation of NOS was found in hepatopancreas and nerve after LPS and poly I-C injection (p < 0 05). These results Suggested that NOS might play an important role in shrimp's defense against pathogenic infection. (C) 2009 Elsevier Ltd All rights reserved
KeywordNos Litopenaeus Vannamei Expression Lps Poly i c
DOI10.1016/j.fsi.2009.12.002
Indexed BySCI
Language英语
WOS IDWOS:000275704900007
Citation statistics
Cited Times:36[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/32842
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Jimei Univ, Fisheries Biotechnol Inst, Coll Fisheries, Fujian Prov Univ,Key Lab Sci & Technol Aquacultur, Xiamen 361021, Peoples R China
Recommended Citation
GB/T 7714
Yao, Cui-Luan,Ji, Pei-Feng,Wang, Zhi-Yong,et al. Molecular cloning and expression of NOS in shrimp, Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(3):453-460.
APA Yao, Cui-Luan,Ji, Pei-Feng,Wang, Zhi-Yong,Li, Fu-hua,&Xiang, Jian-Hai.(2010).Molecular cloning and expression of NOS in shrimp, Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,28(3),453-460.
MLA Yao, Cui-Luan,et al."Molecular cloning and expression of NOS in shrimp, Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 28.3(2010):453-460.
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