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Analysis of metallotionein expression and antioxidant enzyme activities in Meretrix meretrix larvae under sublethal cadmium exposure
Wang, Qing1,3; Wang, Xiaomei2; Wang, Xiaoyu1,4; Yang, Hongsheng1; Liu, Baozhong2
2010-11-15
发表期刊AQUATIC TOXICOLOGY
卷号100期号:4页码:321-328
文章类型Article
摘要To investigate the possible role of metallothioneins (MTs) and antioxidant enzymes in cadmium (Cd) tolerance in Meretrix meretrix larvae, a new MT (designated MmMT) gene was identified and cloned from M. meretrix. The full-length cDNA of MmMT consisted of an open reading frame (ORF) of 231 bp encoding a protein of 76 amino acids, with 21 cysteine residues and a conserved structural pattern Cys-x-Cys-x(3)-Cys-Tyr-Gly-x(3)-Cys-x-Cys-x(3)-CYs-x-Cys-Lys at the C-terminus. The deduced amino acid sequence of MmMT showed about 57-84% identity with previously published MT sequences of mussels and oysters. Real-time PCR was used to analyze the expression level of MmMT mRNA at different M. meretrix larval stages under Cd exposure (25 mu g L(-1)). Results showed that Cd could induce the expression of MmMT mRNA in the larvae, and the expression level increased 5.04-fold and 3.99-fold in D-shaped larvae and pediveligers, respectively. Immunolocalization of MmMT in the stressed larvae revealed that MmMT was synthesized in almost all of the soft parts at the trochophore and postlarva stages, whereas it was only synthesized in the velum and epidermis at the D-shaped larva and pediveliger stages. The activities of three antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), also were measured in larvae at different developmental stages. Increased enzymatic activities were detected mainly in D-shaped larvae and pediveligers under Cd stress, suggesting that these enzymes respond synchronously with MT. Our results indicate that MmMT and antioxidant enzymes played important roles in counteracting Cd stress in M. meretrix larvae. (C) 2010 Elsevier B.V. All rights reserved.
关键词Metallothionein Cadmium Larvae Antioxidant Enzyme Bivalve Meretrix Meretrix
DOI10.1016/j.aquatox.2010.08.012
收录类别SCI
语种英语
WOS记录号WOS:000284787200003
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32831
专题海洋生态与环境科学重点实验室
实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
4.Hebei Univ Technol, Minist Educ, Engn Res Ctr Seawater Utilizat Technol, Tianjin 300130, Peoples R China
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Wang, Qing,Wang, Xiaomei,Wang, Xiaoyu,et al. Analysis of metallotionein expression and antioxidant enzyme activities in Meretrix meretrix larvae under sublethal cadmium exposure[J]. AQUATIC TOXICOLOGY,2010,100(4):321-328.
APA Wang, Qing,Wang, Xiaomei,Wang, Xiaoyu,Yang, Hongsheng,&Liu, Baozhong.(2010).Analysis of metallotionein expression and antioxidant enzyme activities in Meretrix meretrix larvae under sublethal cadmium exposure.AQUATIC TOXICOLOGY,100(4),321-328.
MLA Wang, Qing,et al."Analysis of metallotionein expression and antioxidant enzyme activities in Meretrix meretrix larvae under sublethal cadmium exposure".AQUATIC TOXICOLOGY 100.4(2010):321-328.
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