IOCAS-IR  > 实验海洋生物学重点实验室
Rapid, sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification
Gao Hongwei1,2; Li Fuhua1; Zhang Xiaojun1; Wang Bing1; Xiang Jianhai1
2010
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
Volume28Issue:1Pages:62-66
SubtypeArticle
AbstractVibrio anguillarum is an important bacterial pathogen of aquatic organisms and a significant problem in aquatic farming. The rapid detection and identification of V. anguillarum, and other pathogens that infect marine organisms, is crucial to effective disease management. In this study, we developed a loop-mediated amplification (LAMP) assay to detect V. anguillarum in an hour in a single tube without the need for thermal cycling. Conserved regions of the metalloproteinase (empA) gene of V. anguillarum served as the targets for primer design. A fragment of the empA gene was amplified at 65 degrees C in the presence of the primer mixture and Bst DNA polymerase. In the optimized LAMP assay, 6.7 pg of V. anguillarum DNA could be detected. Six strains of V. anguillarum and 17 strains of non-V. anguillarum bacteria were used in this study to evaluate the species specificity of the primers. The six V. anguillarum strains gave a positive result in the LAMP assay. This method was also validated in V. anguillarum-infected fish. This LAMP method is more sensitive than PCR in the detection of V. anguillarum and shows good species specificity. The LAMP assay is therefore an effective method for the quick detection of V. anguillarum both in the laboratory and in the field.
KeywordLoop-mediated Isothermal Amplification (Lamp) Detection Assay Empa Gene Vibrio Anguillarum
DOI10.1007/s00343-010-9247-3
Indexed BySCI
Language英语
WOS IDWOS:000275654100008
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/32827
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Shandong Entry Exit Inspect & Quarantine Bur Peop, Qingdao 266001, Peoples R China
Recommended Citation
GB/T 7714
Gao Hongwei,Li Fuhua,Zhang Xiaojun,et al. Rapid, sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(1):62-66.
APA Gao Hongwei,Li Fuhua,Zhang Xiaojun,Wang Bing,&Xiang Jianhai.(2010).Rapid, sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(1),62-66.
MLA Gao Hongwei,et al."Rapid, sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.1(2010):62-66.
Files in This Item:
File Name/Size DocType Version Access License
Rapid, sensitive det(511KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Gao Hongwei]'s Articles
[Li Fuhua]'s Articles
[Zhang Xiaojun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Gao Hongwei]'s Articles
[Li Fuhua]'s Articles
[Zhang Xiaojun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Gao Hongwei]'s Articles
[Li Fuhua]'s Articles
[Zhang Xiaojun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Rapid, sensitive detection of Vibrio anguillarum using loop-mediated isothermal amplification.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.