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Construction and Characterization of a Bacterial Artificial Chromosome (BAC) Library of Pacific White Shrimp, Litopenaeus vannamei
Zhang, Xiaojun1; Zhang, Yang1,2,3; Scheuring, Chantel3; Zhang, Hong-Bin3; Huan, Pin1,2; Wang, Bing1; Liu, Chengzhang1; Li, Fuhua1; Liu, Bin1; Xiang, Jianhai1
2010-04-01
发表期刊MARINE BIOTECHNOLOGY
卷号12期号:2页码:141-149
文章类型Article
摘要The pacific white shrimp (Litopenaeus vannamei) is one of the most economically important marine aquaculture species in the world. To facilitate gene cloning and characterization, genome analysis, physical mapping, and molecular selection breeding of marine shrimp, we have developed the techniques to isolate high-quality megabase-sized DNA from hemocyte nuclear DNA of female shrimp and constructed a bacterial artificial chromosome (BAC) genomic library for the species. The library was constructed in the Hind III site of the vector pECBAC1, consisting of 101,760 clones arrayed in 265 384-well microtiter plates, with an average insert size of 101 kb, and covering the genome approximately fivefold. To characterize the library, 92,160 clones were spotted onto high-density nylon filters for hybridization screening. A set of 18 pairs of overgo probes designed from eight cDNA sequences of L. vannamei genes were used in hybridization screening, and 35 positive clones were identified. These results suggest that the shrimp BAC libraries will provide a useful resource for screening of genomic regions of interest candidate genes, gene families, or large-sized synthetic DNA region and promote future works on comparative genomics, physical mapping, and large-scale genome sequencing in the species.
关键词Pacific White Shrimp Litopenaeus Vannamei Bacterial Artificial Chromosome (Bac) Library Genome Overgo Probe
DOI10.1007/s10126-009-9209-y
收录类别SCI
语种英语
WOS记录号WOS:000275754700003
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文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32824
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
3.Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA
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Zhang, Xiaojun,Zhang, Yang,Scheuring, Chantel,et al. Construction and Characterization of a Bacterial Artificial Chromosome (BAC) Library of Pacific White Shrimp, Litopenaeus vannamei[J]. MARINE BIOTECHNOLOGY,2010,12(2):141-149.
APA Zhang, Xiaojun.,Zhang, Yang.,Scheuring, Chantel.,Zhang, Hong-Bin.,Huan, Pin.,...&Xiang, Jianhai.(2010).Construction and Characterization of a Bacterial Artificial Chromosome (BAC) Library of Pacific White Shrimp, Litopenaeus vannamei.MARINE BIOTECHNOLOGY,12(2),141-149.
MLA Zhang, Xiaojun,et al."Construction and Characterization of a Bacterial Artificial Chromosome (BAC) Library of Pacific White Shrimp, Litopenaeus vannamei".MARINE BIOTECHNOLOGY 12.2(2010):141-149.
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