IOCAS-IR  > 实验海洋生物学重点实验室
An immune responsive multidomain galectin from bay scallop Argopectens irradians
Song, Xiaoyan1,2; Zhang, Huan1; Zhao, Jianmin1; Wang, Lingling1; Qiu, Limei1; Mu, Changkao1; Liu, Xiaolin2; Qiu, Lihua3; Song, Linsheng1
2010-02-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号28期号:2页码:326-332
文章类型Article
摘要Galectins are a family of P-galactoside-binding lectins which play crucial roles in innate immunity of vertebrates and invertebrates. In the present study, the cDNA of a galectin with multiple carbohydrate-recognition domains (CRDs) was cloned from bay scallop Argopectens irradians (designated AiGal1) by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) techniques. The full-length cDNA of AiGal1 was of 2235 nucleotides, encoding a polypeptide of 549 amino acids. SMART program analysis revealed that AiGal1 contained four galectin CRDs, and all the CRDs contained the two consensus motifs essential for ligand-binding. Quantitative real-time PCR was employed to investigate the tissue distribution of AiGal1 mRNA and temporal expression in haemocytes of scallops challenged with Vibrio anguillarum, Micrococcus luteus and Pichia pastoris. The AiGal1 mRNA could be detected in all tested tissues with the highest expression level in hepatopancreas. After challenged by V. anguillarum and M. luteus, the expression level of AiGal1 mRNA was both up-regulated and reached the maximum level at 9 h (1.52 fold, P < 0.05) and 18 h (2.89 fold, P < 0.01) post challenge, respectively. However, there was no significant difference in the mRNA expression of AiGal1 in haemocytes after P. pastoris challenge (P > 0.05). These results collectively indicated that AiGal1 was a new member of the galectin family and involved in the immune responses against bacterial infection. (C) 2009 Elsevier Ltd. All rights reserved.
关键词Argopectens Irradians Galectin Mrna Expression Quantitative Real-time Pcr Bacteria And Yeast Challenge
DOI10.1016/j.fsi.2009.11.016
收录类别SCI
语种英语
WOS记录号WOS:000274721300011
引用统计
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32813
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.NW A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China
3.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Song, Xiaoyan,Zhang, Huan,Zhao, Jianmin,et al. An immune responsive multidomain galectin from bay scallop Argopectens irradians[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(2):326-332.
APA Song, Xiaoyan.,Zhang, Huan.,Zhao, Jianmin.,Wang, Lingling.,Qiu, Limei.,...&Song, Linsheng.(2010).An immune responsive multidomain galectin from bay scallop Argopectens irradians.FISH & SHELLFISH IMMUNOLOGY,28(2),326-332.
MLA Song, Xiaoyan,et al."An immune responsive multidomain galectin from bay scallop Argopectens irradians".FISH & SHELLFISH IMMUNOLOGY 28.2(2010):326-332.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
An immune responsive(1165KB)期刊论文出版稿开放获取ODC PDDL浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Song, Xiaoyan]的文章
[Zhang, Huan]的文章
[Zhao, Jianmin]的文章
百度学术
百度学术中相似的文章
[Song, Xiaoyan]的文章
[Zhang, Huan]的文章
[Zhao, Jianmin]的文章
必应学术
必应学术中相似的文章
[Song, Xiaoyan]的文章
[Zhang, Huan]的文章
[Zhao, Jianmin]的文章
相关权益政策
暂无数据
收藏/分享
文件名: An immune responsive multidomain galectin from bay scallop Argopectens irradians.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。