IOCAS-IR  > 实验海洋生物学重点实验室
An immune responsive multidomain galectin from bay scallop Argopectens irradians
Song, Xiaoyan1,2; Zhang, Huan1; Zhao, Jianmin1; Wang, Lingling1; Qiu, Limei1; Mu, Changkao1; Liu, Xiaolin2; Qiu, Lihua3; Song, Linsheng1
2010-02-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume28Issue:2Pages:326-332
SubtypeArticle
AbstractGalectins are a family of P-galactoside-binding lectins which play crucial roles in innate immunity of vertebrates and invertebrates. In the present study, the cDNA of a galectin with multiple carbohydrate-recognition domains (CRDs) was cloned from bay scallop Argopectens irradians (designated AiGal1) by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) techniques. The full-length cDNA of AiGal1 was of 2235 nucleotides, encoding a polypeptide of 549 amino acids. SMART program analysis revealed that AiGal1 contained four galectin CRDs, and all the CRDs contained the two consensus motifs essential for ligand-binding. Quantitative real-time PCR was employed to investigate the tissue distribution of AiGal1 mRNA and temporal expression in haemocytes of scallops challenged with Vibrio anguillarum, Micrococcus luteus and Pichia pastoris. The AiGal1 mRNA could be detected in all tested tissues with the highest expression level in hepatopancreas. After challenged by V. anguillarum and M. luteus, the expression level of AiGal1 mRNA was both up-regulated and reached the maximum level at 9 h (1.52 fold, P < 0.05) and 18 h (2.89 fold, P < 0.01) post challenge, respectively. However, there was no significant difference in the mRNA expression of AiGal1 in haemocytes after P. pastoris challenge (P > 0.05). These results collectively indicated that AiGal1 was a new member of the galectin family and involved in the immune responses against bacterial infection. (C) 2009 Elsevier Ltd. All rights reserved.
KeywordArgopectens Irradians Galectin Mrna Expression Quantitative Real-time Pcr Bacteria And Yeast Challenge
DOI10.1016/j.fsi.2009.11.016
Indexed BySCI
Language英语
WOS IDWOS:000274721300011
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/32813
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.NW A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China
3.Chinese Acad Fishery Sci, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Song, Xiaoyan,Zhang, Huan,Zhao, Jianmin,et al. An immune responsive multidomain galectin from bay scallop Argopectens irradians[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(2):326-332.
APA Song, Xiaoyan.,Zhang, Huan.,Zhao, Jianmin.,Wang, Lingling.,Qiu, Limei.,...&Song, Linsheng.(2010).An immune responsive multidomain galectin from bay scallop Argopectens irradians.FISH & SHELLFISH IMMUNOLOGY,28(2),326-332.
MLA Song, Xiaoyan,et al."An immune responsive multidomain galectin from bay scallop Argopectens irradians".FISH & SHELLFISH IMMUNOLOGY 28.2(2010):326-332.
Files in This Item:
File Name/Size DocType Version Access License
An immune responsive(1165KB)期刊论文出版稿开放获取ODC PDDLView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Song, Xiaoyan]'s Articles
[Zhang, Huan]'s Articles
[Zhao, Jianmin]'s Articles
Baidu academic
Similar articles in Baidu academic
[Song, Xiaoyan]'s Articles
[Zhang, Huan]'s Articles
[Zhao, Jianmin]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Song, Xiaoyan]'s Articles
[Zhang, Huan]'s Articles
[Zhao, Jianmin]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: An immune responsive multidomain galectin from bay scallop Argopectens irradians.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.