| Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals | |
| Liu Jianguo1; Zhang Xiaoli1,2; Sun Yanhong1,2; Lin Wei1 | |
| 2010 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 卷号 | 28期号:1页码:1-9 |
| 文章类型 | Article |
| 摘要 | The antioxidative capacity of astaxanthin and enzyme activity of reactive oxygen eliminating enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) were studied in three cell types of Haematococcus pluvialis exposed to high concentrations of a superoxide anion radical (O(2)(-)). The results show that defensive enzymes and astaxanthin-related mechanisms were both active in H. pluvialis during exposure to reactive oxygen species (ROS) such as O(2)(-) . Astaxanthin reacted with ROS much faster than did the protective enzymes, and had the strongest antioxidative capacity to protect against lipid peroxidation. The defensive mechanisms varied significantly between the three cell types and were related to the level of astaxanthin that had accumulated in those cells. Astaxanthin-enriched red cells had the strongest antioxidative capacity, followed by brown cells, and astaxanthin-deficient green cells. Although there was no significant increase in expression of protective enzymes, the malondialdehyde (MDA) content in red cells was sustained at a low level because of the antioxidative effect of astaxanthin, which quenched O(2)(-) before the protective enzymes could act. In green cells, astaxanthin is very low or absent; therefore, scavenging of ROS is inevitably reliant on antioxidative enzymes. Accordingly, in green cells, these enzymes play the leading role in scavenging ROS, and the expression of these enzymes is rapidly increased to reduce excessive ROS. However, because ROS were constantly increased in this study, the enhance enzyme activity in the green cells was not able to repair the ROS damage, leading to elevated MDA content. Of the four defensive enzymes measured in astaxanthin-deficient green cells, SOD eliminates O(2)(-), POD eliminates H(2)O(2), which is a by-product of SOD activity, and APX and CAT are then initiated to scavenge excessive ROS. |
| 关键词 | Astaxanthin Haematococcus Pluvialis Lipid Peroxidation Reactive Oxygen Species (Ros) |
| DOI | 10.1007/s00343-010-9244-6 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000275654100001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32797 |
| 专题 | 海洋生物技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Res & Dev Ctr Marine Biotechnol, Qingdao 260071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Liu Jianguo,Zhang Xiaoli,Sun Yanhong,et al. Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(1):1-9. |
| APA | Liu Jianguo,Zhang Xiaoli,Sun Yanhong,&Lin Wei.(2010).Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(1),1-9. |
| MLA | Liu Jianguo,et al."Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.1(2010):1-9. |
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