Identification and molecular analysis of a ferritin subunit from red drum (Sciaenops ocellatus)

doi:10.1016/j.fsi.2010.01.001

IOCAS-IR > 实验海洋生物学重点实验室

	Identification and molecular analysis of a ferritin subunit from red drum (Sciaenops ocellatus)
	Hu, Yong-hua 1; Zheng, Wen-jiang 1,2; Sun, Li 1
	2010-04-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	28 期号:4 页码:678-686
文章类型	Article
摘要	Ferritin is a conserved iron binding protein existing ubiquitously in prokaryotes and eukaryotes. In this study, the gene encoding a ferritin M subunit homologue (SoFer1) was cloned from red drum (Sciaenops ocellatus) and analyzed at expression and functional levels. The open reading frame of SoFer1 is 531 bp and preceded by a 5'-untranslated region that contains a putative Iron Regulatory Element (IRE) preserved in many ferritins. The deduced amino acid sequence of SoFer1 possesses both the ferroxidase center of mammalian H ferritin and the iron nucleation site of mammalian L ferritin. Expression of SoFer1 was tissue specific and responded positively to experimental challenges with Gram-positive and Gram-negative fish pathogens. Treatment of red drum liver cells with iron, copper, and oxidant significantly upregulated the expression of SoFer1 in time-dependent manners. To further examine the potential role of SoFer1 in antioxidation, red drum liver cells transfected transiently with SoFer1 were prepared. Compared to control cells, SoFer1 transfectants exhibited reduced production of reactive oxygen species following H(2)O(2) challenge. Finally, to examine the iron binding potential of SoFer1. SoFer1 was expressed in and purified from Escherichia colt as a recombinant protein. Iron-chelating analysis showed that purified recombinant SoFer1 was capable of iron binding. Taken together, these results suggest that SoFer1 is likely to be a functional ferritin involved in iron sequestration, host immune defence against bacterial infection, and antioxidation. (C) 2010 Elsevier Ltd. All rights reserved.
关键词	Ferritin Immune Defence Iron Oxidative Stress Sciaenops Ocellatus
DOI	10.1016/j.fsi.2010.01.001
收录类别	SCI
语种	英语
WOS记录号	WOS:000276935700022
引用统计	被引频次：107[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32774
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Hu, Yong-hua,Zheng, Wen-jiang,Sun, Li. Identification and molecular analysis of a ferritin subunit from red drum (Sciaenops ocellatus)[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(4):678-686.
APA	Hu, Yong-hua,Zheng, Wen-jiang,&Sun, Li.(2010).Identification and molecular analysis of a ferritin subunit from red drum (Sciaenops ocellatus).FISH & SHELLFISH IMMUNOLOGY,28(4),678-686.
MLA	Hu, Yong-hua,et al."Identification and molecular analysis of a ferritin subunit from red drum (Sciaenops ocellatus)".FISH & SHELLFISH IMMUNOLOGY 28.4(2010):678-686.

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文件名称/大小	文献类型	版本类型	开放类型	使用许可
Identification and m（1065KB）	期刊论文	出版稿	限制开放	ODC PDDL	浏览