Effects of cell density, light intensity and mixing on Undaria pinnatifida gametophyte activity in a photobioreactor

doi:10.1016/S1389-0344(03)00071-6

IOCAS-IR > 实验海洋生物学重点实验室

	Effects of cell density, light intensity and mixing on Undaria pinnatifida gametophyte activity in a photobioreactor
	Zou, M ; Zhou, BC ; Li, BJ ; Sun, DH ; Zeng, CK
	2003-07-01
发表期刊	BIOMOLECULAR ENGINEERING
ISSN	1389-0344
卷号	20 期号:4-6 页码:281-284
文章类型	Article
摘要	An on-line controlled 7 1 sterilizable photobioreactor was used for the optimisation of a culture of gametophytes of Undaria pinnatifida. The gametophytes, which had been stored for three years in a culture cabinet at 16 degreesC, could rapidly grow in the photobioreactor under controlled conditions. The rate of increase of dissolved oxygen and pH were used to monitor the photosynthetic activity. Optimal gametophytes density changed varying the light intensity. The optimal cell densities were 3.24 and 3.45 g FW l(-1) when the cultures were exposed to 61.7 and 82.3 muE m(-2) s(-1), respectively. The optimal cell density was higher under a high photon flux density (PFD) than under low PFD. On the other hand, the optimal light intensities were different for different cell density cultures. The light saturation point was higher at high cell density cultures than at low cell density cultures. The optimal rotational speed was 150 rpm for high cell density culture in the photobioreactor. (C) 2003 Elsevier B.V. All rights reserved.; An on-line controlled 7 1 sterilizable photobioreactor was used for the optimisation of a culture of gametophytes of Undaria pinnatifida. The gametophytes, which had been stored for three years in a culture cabinet at 16 degreesC, could rapidly grow in the photobioreactor under controlled conditions. The rate of increase of dissolved oxygen and pH were used to monitor the photosynthetic activity. Optimal gametophytes density changed varying the light intensity. The optimal cell densities were 3.24 and 3.45 g FW l(-1) when the cultures were exposed to 61.7 and 82.3 muE m(-2) s(-1), respectively. The optimal cell density was higher under a high photon flux density (PFD) than under low PFD. On the other hand, the optimal light intensities were different for different cell density cultures. The light saturation point was higher at high cell density cultures than at low cell density cultures. The optimal rotational speed was 150 rpm for high cell density culture in the photobioreactor. (C) 2003 Elsevier B.V. All rights reserved.
关键词	Gametophytes Light Intensity Cell Density Mixing Undaria Pinnatifida Photosynthetic Activity
学科领域	Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Genetics & Heredity
DOI	10.1016/S1389-0344(03)00071-6
URL	查看原文
收录类别	ISTP ; SCI
语种	英语
WOS记录号	WOS:000184977900023
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3276
专题	实验海洋生物学重点实验室
作者单位	1.Yantai Teachers Coll, Biol Dept, Yantai 264025, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Yantai Univ, Ocean Coll, Yantai 264005, Peoples R China
推荐引用方式 GB/T 7714	Zou, M,Zhou, BC,Li, BJ,et al. Effects of cell density, light intensity and mixing on Undaria pinnatifida gametophyte activity in a photobioreactor[J]. BIOMOLECULAR ENGINEERING,2003,20(4-6):281-284.
APA	Zou, M,Zhou, BC,Li, BJ,Sun, DH,&Zeng, CK.(2003).Effects of cell density, light intensity and mixing on Undaria pinnatifida gametophyte activity in a photobioreactor.BIOMOLECULAR ENGINEERING,20(4-6),281-284.
MLA	Zou, M,et al."Effects of cell density, light intensity and mixing on Undaria pinnatifida gametophyte activity in a photobioreactor".BIOMOLECULAR ENGINEERING 20.4-6(2003):281-284.

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