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Isolation and characterization of lethal proteins in nematocyst venom of the jellyfish Cyanea nozakii Kishinouye
Feng, Jinhua1,2; Yu, Huahua1; Li, Cuiping1,2; Xing, Ronge1; Liu, Song1; Wang, Lin1,2; Cai, Shengbao1,2; Li, Pengcheng1
2010
发表期刊TOXICON
卷号55期号:1页码:118-125
文章类型Article
摘要Cyanea nozakii Kishinouye, a jellyfish widely distributed in coastal areas of China, has garnered attention because of its stinging capacity and the resulting public health hazard. We used a recently developed technique to extract jellyfish venom from nematocysts; the present study investigates the lethality of C. nozakii venom. The nematocyst contents were extremely toxic to the grass carp, Ctenopharyngodon idellus, producing typical neurotoxin toxicity. The ID50 was about 0.6 mu g protein/g fish. Toxin samples were stable when kept at -80 degrees C, but after 48 h, an 80% decline in lethality occurred at -20 degrees C. Poor stability of the venom was observed within the range of 65-80 degrees C and at pH 3.5. The venom was hydrolyzed by a proteolytic enzyme, trypsin. Fractionation of the venom yielded two protein bands with molecular weights of 60 kDa and 50 kDa. Our results provide the first evidence that C. nozakii produces lethal toxins. These characteristics highlight the need for the isolation and molecular characterization of new active toxins in C nozakii. (C) 2009 Elsevier Ltd. All rights reserved.
关键词Jellyfish Cyanea Nozakii Kishinouye Nematocyst Lethality Isolation
DOI10.1016/j.toxicon.2009.07.008
收录类别SCI
语种英语
WOS记录号WOS:000273921500015
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32769
专题海洋生物技术研发中心
实验海洋生物学重点实验室
海洋环境工程技术研究发展中心
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
第一作者单位中国科学院海洋研究所
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Feng, Jinhua,Yu, Huahua,Li, Cuiping,et al. Isolation and characterization of lethal proteins in nematocyst venom of the jellyfish Cyanea nozakii Kishinouye[J]. TOXICON,2010,55(1):118-125.
APA Feng, Jinhua.,Yu, Huahua.,Li, Cuiping.,Xing, Ronge.,Liu, Song.,...&Li, Pengcheng.(2010).Isolation and characterization of lethal proteins in nematocyst venom of the jellyfish Cyanea nozakii Kishinouye.TOXICON,55(1),118-125.
MLA Feng, Jinhua,et al."Isolation and characterization of lethal proteins in nematocyst venom of the jellyfish Cyanea nozakii Kishinouye".TOXICON 55.1(2010):118-125.
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