Development of gene-targeted SNP markers for genomic mapping in Pacific abalone Haliotis discus hannai Ino

doi:10.1007/s11033-010-0032-3

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	Development of gene-targeted SNP markers for genomic mapping in Pacific abalone Haliotis discus hannai Ino
	Qi, Haigang; Liu, Xiao; Wu, Fucun; Zhang, Guofan
	2010-12-01
发表期刊	MOLECULAR BIOLOGY REPORTS
卷号	37 期号:8 页码:3779-3784
文章类型	Article
摘要	Useful and novel DNA markers are needed for aquaculture genetics and breeding. In this study, we report the discovery and development of gene-targeted single nucleotide polymorphisms (SNPs) for genomic mapping in the Pacific abalone Haliotis discus hannai Ino. Single EST or EST-contigs from 66 genes that had positive BLASTx matches (E-value a parts per thousand currency sign 1e-8) were used for polymerase chain reaction (PCR) amplification. PCR products from the two parents of one mapping family were directly sequenced, and 83 SNP loci were found from 17 genes. Allele-specific PCR (AS-PCR) was developed and optimized for genotyping of 11 SNP loci in 120 progeny of the mapping family. Nine of the loci conformed to the expected Mendelian ratio of 1:1 based on the chi(2) test (P > 0.05) and could potentially be used for linkage map construction. Our data also indicate that the sequencing of two parents may be a practical strategy for the discovery of informative SNPs for linkage mapping in a particular mapping population.
关键词	Snps Pacific Abalone Haliotis Discus Hannai Ino Direct Sequencing As-pcr
DOI	10.1007/s11033-010-0032-3
收录类别	SCI
语种	英语
WOS记录号	WOS:000283577400016
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32760
专题	海洋生物技术研发中心
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Qi, Haigang,Liu, Xiao,Wu, Fucun,et al. Development of gene-targeted SNP markers for genomic mapping in Pacific abalone Haliotis discus hannai Ino[J]. MOLECULAR BIOLOGY REPORTS,2010,37(8):3779-3784.
APA	Qi, Haigang,Liu, Xiao,Wu, Fucun,&Zhang, Guofan.(2010).Development of gene-targeted SNP markers for genomic mapping in Pacific abalone Haliotis discus hannai Ino.MOLECULAR BIOLOGY REPORTS,37(8),3779-3784.
MLA	Qi, Haigang,et al."Development of gene-targeted SNP markers for genomic mapping in Pacific abalone Haliotis discus hannai Ino".MOLECULAR BIOLOGY REPORTS 37.8(2010):3779-3784.

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