DNA adenine methylase is involved in the pathogenesis of Edwardsiella tarda

doi:10.1016/j.vetmic.2009.09.004

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	DNA adenine methylase is involved in the pathogenesis of Edwardsiella tarda
	Sun, Kun 1,2; Jiao, Xu-dong 1,3; Zhang, Min 1; Sun, Li 1
	2010-02-24
发表期刊	VETERINARY MICROBIOLOGY
卷号	141 期号:1-2 页码:149-154
文章类型	Article
摘要	Edwardsiella tarda is a serious aquaculture pathogen that can infect many Cultured fish species. The aim of this study was to investigate the potential importance of DNA adenine methylase (Dam) in E. tarda pathogenesis. The E. tardo dam gene (dam(Et)) was cloned from a pathogenic strain, TXD1, isolated from diseased fish. Dam(Et) shares high (70.2%) sequence identity with the Dam proteins of Yersinia enterocolitica and several other bacterial species. Recombinant Dam(Et) is able to complement a dam-deficient Escherichia coli strain and methylate the genomic DNA. Attenuation of dam(Et) expression by antisense RNA interference had no apparent effect on the growth of TXD1, but caused significant attenuation of overall bacterial virulence and altered several stress responses including spontaneous mutation, recovering from UV radiation and H(2)O(2) exposure, binding to host mucus, and dissemination in host blood and liver. In addition, attenuation of dam(Et) expression increased luxS expression and AI-2 activities in E. tarda. These results indicate that Dam(Et) is a virulence determinant and plays a role in the pathogenesis of TXD1, and that temporal expression of dam(Et), is essential for optimal bacterial infection. (C) 2009 Elsevier B.V. All rights reserved.
关键词	Antisense Rna Dna Adenine Methylase Edwardsiella Tarda Rna Interference Virulence
DOI	10.1016/j.vetmic.2009.09.004
收录类别	SCI
语种	英语
WOS记录号	WOS:000275610200022
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32755
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Polar Res Inst China, Key Lab Polar Sci, State Ocean Adm, Shanghai 200136, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun, Kun,Jiao, Xu-dong,Zhang, Min,et al. DNA adenine methylase is involved in the pathogenesis of Edwardsiella tarda[J]. VETERINARY MICROBIOLOGY,2010,141(1-2):149-154.
APA	Sun, Kun,Jiao, Xu-dong,Zhang, Min,&Sun, Li.(2010).DNA adenine methylase is involved in the pathogenesis of Edwardsiella tarda.VETERINARY MICROBIOLOGY,141(1-2),149-154.
MLA	Sun, Kun,et al."DNA adenine methylase is involved in the pathogenesis of Edwardsiella tarda".VETERINARY MICROBIOLOGY 141.1-2(2010):149-154.

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