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Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology
Zhang, YZ; Zhang, SC; Liu, XZ; Xu, YY; Wang, CL; Sawant, MS; Li, J; Chen, SL
2003-09-15
发表期刊THERIOGENOLOGY
ISSN0093-691X
卷号60期号:5页码:989-996
文章类型Article
摘要A simple and convenient protocol for the cryopreservation of the flounder (Paralichthys olivaceus) sperm was established for "on the spot" cryopreservation of large quantities of semen. The use of three cryoprotectants, dimethyl sulphoxide (DMSO), glycerol (Gly) and methanol was tested in the method. The percentage of motile sperm present in semen after it had been frozen and thawed in the presence of DMSO, Gly or methanol was 60.5 +/- 3.6, 79.17 +/- 4.5 and 13.25 +/- 4.7%, respectively. The fertilization rates of this sperm were 67.06 +/- 15.1, 76.20 +/- 10.0 and 44.93 +/- 22.6%, while the hatching rates of eggs fertilized with this sperm were 37.40 +/- 8.3, 48.18 +/- 25.7 and 23.35 +/- 10.8%, respectively. It was found that Gly and DMSO were better cryoprotectants than methanol, with Gly giving the best overall results. Under scanning electron microscopy, it could be seen that while the majority of the frozen-thawed sperm remained morphologically normal, some exhibited lost or dilated mitochondria, swollen mid-pieces, broken tails, or damaged cell membrane, which probably caused the decrease in motility and fertility of the frozen-thawed sperm. (C) 2003 Elsevier Science Inc. All rights reserved.; A simple and convenient protocol for the cryopreservation of the flounder (Paralichthys olivaceus) sperm was established for "on the spot" cryopreservation of large quantities of semen. The use of three cryoprotectants, dimethyl sulphoxide (DMSO), glycerol (Gly) and methanol was tested in the method. The percentage of motile sperm present in semen after it had been frozen and thawed in the presence of DMSO, Gly or methanol was 60.5 +/- 3.6, 79.17 +/- 4.5 and 13.25 +/- 4.7%, respectively. The fertilization rates of this sperm were 67.06 +/- 15.1, 76.20 +/- 10.0 and 44.93 +/- 22.6%, while the hatching rates of eggs fertilized with this sperm were 37.40 +/- 8.3, 48.18 +/- 25.7 and 23.35 +/- 10.8%, respectively. It was found that Gly and DMSO were better cryoprotectants than methanol, with Gly giving the best overall results. Under scanning electron microscopy, it could be seen that while the majority of the frozen-thawed sperm remained morphologically normal, some exhibited lost or dilated mitochondria, swollen mid-pieces, broken tails, or damaged cell membrane, which probably caused the decrease in motility and fertility of the frozen-thawed sperm. (C) 2003 Elsevier Science Inc. All rights reserved.
关键词Flounder Paralichthys Olivaceus Sperm Cryopreservation Glycerol Dimethyl Sulphoxide Methanol
学科领域Reproductive Biology ; Veterinary Sciences
DOI10.1016/S0093-691X(03)00097-9
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000185216600018
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被引频次:74[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/3274
专题实验海洋生物学重点实验室
作者单位1.Ocean Univ Qingdao, Dept Marine Biol, Qingdao 266003, Peoples R China
2.Liaocheng Univ, Dept Life Sci, Liaocheng 252059, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
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GB/T 7714
Zhang, YZ,Zhang, SC,Liu, XZ,et al. Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology[J]. THERIOGENOLOGY,2003,60(5):989-996.
APA Zhang, YZ.,Zhang, SC.,Liu, XZ.,Xu, YY.,Wang, CL.,...&Chen, SL.(2003).Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology.THERIOGENOLOGY,60(5),989-996.
MLA Zhang, YZ,et al."Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology".THERIOGENOLOGY 60.5(2003):989-996.
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