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EseD, a Putative T3SS Translocon Component of Edwardsiella tarda, Contributes to Virulence in Fish and is a Candidate for Vaccine Development
Wang, Bo; Mo, Zhao Lan; Xiao, Peng; Li, Jie; Zou, Yu Xia; Hao, Bin; Li, Gui Yang
2010-11-01
发表期刊MARINE BIOTECHNOLOGY
卷号12期号:6页码:678-685
文章类型Article
摘要Edwardsiella tarda has a type III secretion system (T3SS) essential for pathogenesis. EseD, together with EseB and EseC, has been suggested to form a putative T3SS translocon complex, although its further function is unclear. To investigate the physiological role of EseD, a mutant strain of E. tarda was constructed with an in-frame deletion of the entire eseD gene. One finding was that the a dagger eseD mutant decreased the secretion levels of EseC and EseB proteins. Additionally, the a dagger eseD mutant showed attenuated swarming and contact-hemolysis abilities. However, the a dagger eseD mutant showed increased biofilm formation. Complementation of the mutant strain with eseD restored these phenotypes to those similar to the wild-type strain. Furthermore, infection experiments in fish showed that the a dagger eseD mutant exhibited slower proliferation and a tenfold decrease in virulence in fish. These results indicate a specific role of EseD in the pathogenesis of E. tarda. Finally, recombinant EseD protein elicited high antibody titers in immunized fish and various levels of protection against lethal challenge with the wild-type strain. These results indicate that EseD protein may be a candidate antigen for development of a subunit vaccine against Edwardsiellosis.
关键词E. Tarda Esed Mutant Pathogenesis Recombinant Esed Protective Immunity
DOI10.1007/s10126-009-9255-5
收录类别SCI
语种英语
WOS记录号WOS:000284599000006
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被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32740
专题实验海洋生物学重点实验室
海洋生物分类与系统演化实验室
作者单位Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
第一作者单位实验海洋生物学重点实验室
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Wang, Bo,Mo, Zhao Lan,Xiao, Peng,et al. EseD, a Putative T3SS Translocon Component of Edwardsiella tarda, Contributes to Virulence in Fish and is a Candidate for Vaccine Development[J]. MARINE BIOTECHNOLOGY,2010,12(6):678-685.
APA Wang, Bo.,Mo, Zhao Lan.,Xiao, Peng.,Li, Jie.,Zou, Yu Xia.,...&Li, Gui Yang.(2010).EseD, a Putative T3SS Translocon Component of Edwardsiella tarda, Contributes to Virulence in Fish and is a Candidate for Vaccine Development.MARINE BIOTECHNOLOGY,12(6),678-685.
MLA Wang, Bo,et al."EseD, a Putative T3SS Translocon Component of Edwardsiella tarda, Contributes to Virulence in Fish and is a Candidate for Vaccine Development".MARINE BIOTECHNOLOGY 12.6(2010):678-685.
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