Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| A putative endocrine factor SIBD (single insulin binding domain protein) involved in immune response of Chinese mitten crab Eriocheir sinensis | |
| Gai, Yunchao1,2; Wang, Lingling1; Song, Linsheng1; Zhao, Jianmin1; Qiu, Limei1; Xing, Kezhi3 | |
| 2010 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
 |
| 卷号 | 28期号:1页码:10-17 |
| 文章类型 | Article |
| 摘要 | Insulin growth factor binding proteins (IGFBPs), characterized by the conserved insulin binding (IB) domains, are important components of endocrine system and play key roles in metabolism and growth. In the present study, the full-length cDNA of a single IB domain protein (designated EsSIBD) was identified from Chinese mitten crab Erlocheir sinensis based on expressed sequence tag (EST) sequence. The 1187 bp EsSIBD cDNA contained a 321 bp open reading frame (ORF) encoding a putative protein of 106 amino acids, a T-untranslated region (UTR) of 189 bp, and a 3'-UTR of 677 bp. Multiple sequence alignment presented ten conserved cysteine residues critical for the fundamental structure and function of IB domain. BLAST analysis revealed that EsSIBD shared high similarity with previously known IB domains of IGFBPs; with the identities ranging from 40% to 46%. The sequence similarity and domain conservation indicated that EsSIBD was a potential member of the IGFBP family. Phylogenic analysis presented that EsSIBD was closer to IGFBP7 than to the other 113 domain containing proteins, suggesting its functional similarity with the endocrine factor IFGBP7. The mRNA expression of EsSIBD in different tissues including hepatopancreas, gill, gonad, muscle, heart and haemocytes, and the temporal expression in haemocytes challenged by Listonella anguillarum were measured by real-time RT-1 CR. EsSIBD mRNA transcripts could be detected in all examined tissues with the highest expression level in gill. The EsSIBD mRNA expression in haemocytes was sensitive to L anguillarum stimulation and it was up-regulated from 3 to 24 h after challenge. The structure conservation and functional similarity to IFGBPs, and its sensitivity to L anguillarum stimulation collectively implied that EsSIBD was probably involved in endocrine and immune systems of Chinese mitten crab, and provided insight into the cross-talk between the invertebrate endocrine and immune system. (C) 2009 Elsevier Ltd. All rights reserved. |
| 关键词 | Igfbp Insulin Binding Domain Eriocheir Sinensis Arthropod Endocrine Innate Immunity Mrna Expression Profile |
| DOI | 10.1016/j.fsi.2009.09.013 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000274235100002 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32738 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Tianjin Agr Coll, Tianjin 300381, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Gai, Yunchao,Wang, Lingling,Song, Linsheng,et al. A putative endocrine factor SIBD (single insulin binding domain protein) involved in immune response of Chinese mitten crab Eriocheir sinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(1):10-17. |
| APA | Gai, Yunchao,Wang, Lingling,Song, Linsheng,Zhao, Jianmin,Qiu, Limei,&Xing, Kezhi.(2010).A putative endocrine factor SIBD (single insulin binding domain protein) involved in immune response of Chinese mitten crab Eriocheir sinensis.FISH & SHELLFISH IMMUNOLOGY,28(1),10-17. |
| MLA | Gai, Yunchao,et al."A putative endocrine factor SIBD (single insulin binding domain protein) involved in immune response of Chinese mitten crab Eriocheir sinensis".FISH & SHELLFISH IMMUNOLOGY 28.1(2010):10-17. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| A putative endocrine（722KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
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