Direct effects of fascaplysin on human umbilical vein endothelial cells attributing the anti-angiogenesis activity

doi:10.1016/j.biopha.2009.04.046

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	Direct effects of fascaplysin on human umbilical vein endothelial cells attributing the anti-angiogenesis activity
	Zheng, Y. L.3; Lu, X. L.1; Lin, J.1; Chen, H. M.1; Yan, X. J.1; Wang, F.2 ; Xu, W. F.2
	2010-10-01
发表期刊	BIOMEDICINE & PHARMACOTHERAPY
卷号	64 期号:8 页码:527-533
文章类型	Article
摘要	Novel anti-angiogenesis activity of fascaplysin via VEGF blockage was recently revealed by our previous study in addition to the reported cyclin-dependent kinase 4 (CDK4) selective inhibition. To uncover more details of this pharmacologically prospective property, this study further investigated whether fascaplysin had direct anti-proliferation effects on human umbilical vein endothelial cells (HUVEC), which might be contributing to anti-angiogenesis. The results showed that Cl cell cycle arrest was induced by 2.6 mu M fascaplysin in a time-dependent manner, and exhibited more sensitive than hepatocarcinoma cells BeL-7402 and Hela cells. Approximately 56.09 +/- 2.63% of the cells were arrested at the Cl phase after 24 h, and 64.94 +/- 2.07% after 36 h, comparing to the 22.82 +/- 1.2% in methanol treated cells. Apoptosis of HUVEC cells was induced by 1.3 mu M fascaplysin and indicated by the sub-G1, Hoechst staining, terminal deoxynucleotidyl transferase dUTP-mediated nicked end labeling (TUNEL) assay, and annexin-V and propidium (PI) label. This apoptosis response was further confirmed by the detection of active caspase-3 and by western blotting using antibodies against Bax, Bcl-2, procaspase-8, and Bid, indicating that apoptosis in HUVEC cells may involve a mitochondria pathway, by the demonstration of an increase in the Bax/Bcl-2 ratio. Together, our results suggest that the anti-angiogenesis activity of fascaplysin is through the direct effects of cell cycle arrest and apoptosis on HUVEC. (C) 2009 Elsevier Masson SAS. All rights reserved.
关键词	Fascaplysin Anti-angiogenesis Huvec G1 Cell Cycle Arrest Apoptosis
DOI	10.1016/j.biopha.2009.04.046
收录类别	SCI
语种	英语
WOS记录号	WOS:000283571100004
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32730
专题	海洋生物技术研发中心海洋生态与环境科学重点实验室
作者单位	1.Ningbo Univ, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo, Zhejiang, Peoples R China 2.Lihuili Hosp, Ningbo Med Ctr, Dept Clin Lab, Ningbo 315041, Zhejiang, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zheng, Y. L.,Lu, X. L.,Lin, J.,et al. Direct effects of fascaplysin on human umbilical vein endothelial cells attributing the anti-angiogenesis activity[J]. BIOMEDICINE & PHARMACOTHERAPY,2010,64(8):527-533.
APA	Zheng, Y. L..,Lu, X. L..,Lin, J..,Chen, H. M..,Yan, X. J..,...&Xu, W. F..(2010).Direct effects of fascaplysin on human umbilical vein endothelial cells attributing the anti-angiogenesis activity.BIOMEDICINE & PHARMACOTHERAPY,64(8),527-533.
MLA	Zheng, Y. L.,et al."Direct effects of fascaplysin on human umbilical vein endothelial cells attributing the anti-angiogenesis activity".BIOMEDICINE & PHARMACOTHERAPY 64.8(2010):527-533.

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