Analysis of the vaccine potential of a laboratory Escherichia coli strain in a Japanese flounder model

doi:10.1016/j.fsi.2009.11.003

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	Analysis of the vaccine potential of a laboratory Escherichia coli strain in a Japanese flounder model
	Cheng, Shuang 1,2; Jiao, Xu-dong 1,2; Zhang, Min 1,2; Sun, Li 1
	2010-02-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
卷号	28 期号:2 页码:275-280
文章类型	Article
摘要	Escherichia coli DH5 alpha is a genetically tailored laboratory strain that is commonly used for general cloning. In this study, the vaccine potential of DH5 alpha was investigated. It was found that when used as a live vaccine, DH5 alpha could afford effective protection upon Japanese flounder against Aeromonas hydrophila infection. Vaccination with purified outer membrane proteins and lipopolysaccharides of DH5 alpha failed to induce protective immunity against A. hydrophila. Specific antibody production was observed in fish immunized with DH5 alpha, which lasted at least 8 weeks and was enhanced by a booster injection during the vaccination process. Analysis of the transcription profiles of immune-related genes showed that vaccination with DH5 alpha heightened the expression of the genes encoding factors that are likely involved in both specific and nonspecific immunities. Furthermore, compared to the control fish, fish vaccinated with DH5 alpha/pAQ1, which is DH5 alpha harboring the plasmid pAQ1 that expresses the coding element of a Vibrio harveyi antigen, exhibited significantly improved survival rates following V harveyi and A. hydrophila challenges. These results demonstrate that DH5 alpha possesses intrinsic immunoprotective potential against A. hydrophila. This property, together with the feature of cloning friendliness, should render DH5 alpha useful in the construction of cross-protective vaccines. (C) 2009 Elsevier Ltd. All rights reserved.
关键词	Aeromonas Hydrophila Cross-protection Dh5 Alpha Escherichia Coli Vibrio Harveyi
DOI	10.1016/j.fsi.2009.11.003
收录类别	SCI
语种	英语
WOS记录号	WOS:000274721300004
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32716
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Cheng, Shuang,Jiao, Xu-dong,Zhang, Min,et al. Analysis of the vaccine potential of a laboratory Escherichia coli strain in a Japanese flounder model[J]. FISH & SHELLFISH IMMUNOLOGY,2010,28(2):275-280.
APA	Cheng, Shuang,Jiao, Xu-dong,Zhang, Min,&Sun, Li.(2010).Analysis of the vaccine potential of a laboratory Escherichia coli strain in a Japanese flounder model.FISH & SHELLFISH IMMUNOLOGY,28(2),275-280.
MLA	Cheng, Shuang,et al."Analysis of the vaccine potential of a laboratory Escherichia coli strain in a Japanese flounder model".FISH & SHELLFISH IMMUNOLOGY 28.2(2010):275-280.

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