Rapid methods for extracting high-quality DNA from shell-boring "Conchocelis''

doi:10.1515/BOT.2010.007

IOCAS-IR > 实验海洋生物学重点实验室

	Rapid methods for extracting high-quality DNA from shell-boring "Conchocelis''
	Wang, Jinfeng1,2 ; Jiang, Peng 1; Zhou, Wei 3; Cui, Yulin 1,2; Qin, Song 1
	2010-02-01
发表期刊	BOTANICA MARINA
卷号	53 期号:1 页码:63-68
文章类型	Article
摘要	The genome DNA required for molecular studies of Porphyra has generally been extracted from either blades or free-living "Conchocelis''. However, the main disadvantages are complicated procedures, high cost, and DNA contamination. In this study, we developed a strategy for pure DNA extraction from shell-boring "Conchocelis'' and a single-step technique to prepare PCR templates. The results show that contamination of free-living "Conchocelis'' with microalgae was eliminated after the filaments had bored into shells. The electrophoretic banding patterns and intensities were similar between materials obtained from free-living "Conchocelis'' and shell-boring "Conchocelis'', suggesting that total DNA extraction from the latter was efficient. Molecular evidence demonstrated additional bands in PCR products of internal transcribed spacer (ITS) amplifications prepared from cultures of free-living "Conchocelis'' contaminated with microalgae. The additional bands appeared in all three Porphyra yezoensis replicates and one out of three Porphyra haitanensis replicates, but were not detectable in preparations from shell-boring "Conchocelis''. The DNA extracted from shell-boring "Conchocelis'' reduced the risk of microalgal contamination and the single-step technique reduced the time required for the preparation of DNA for molecular studies of Porphyra. The single-step method should be useful for PCR amplification of small amounts of materials, large sample sizes, or samples contaminated with microalgae. The technique may also be suitable for other genera of Bangiaceae, providing that shell-boring "Conchocelis'' stages are present in their life cycles.
关键词	Dna Extraction Its Porphyra Rbcl Shell-boring "conchocelis''
DOI	10.1515/BOT.2010.007
收录类别	SCI
语种	英语
WOS记录号	WOS:000274192000006
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/32661
专题	实验海洋生物学重点实验室海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Marine Fisheries Inst Jiangsu, Nantong 226007, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Jinfeng,Jiang, Peng,Zhou, Wei,et al. Rapid methods for extracting high-quality DNA from shell-boring "Conchocelis''[J]. BOTANICA MARINA,2010,53(1):63-68.
APA	Wang, Jinfeng,Jiang, Peng,Zhou, Wei,Cui, Yulin,&Qin, Song.(2010).Rapid methods for extracting high-quality DNA from shell-boring "Conchocelis''.BOTANICA MARINA,53(1),63-68.
MLA	Wang, Jinfeng,et al."Rapid methods for extracting high-quality DNA from shell-boring "Conchocelis''".BOTANICA MARINA 53.1(2010):63-68.

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