Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Cyclic AMP concentration and protein kinase a (PKA) gene expression at different developmental stages of the polychaete Hydroides elegans | |
Li, Hong-Lei1,2 ; Song, Lin-Sheng1,2,3; Qian, Pei-Yuan1,2
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| 2008-07-15 | |
| 发表期刊 | JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
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| 卷号 | 310B期号:5页码:417-427 |
| 文章类型 | Article |
| 摘要 | The phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) showed inductive effect on larval settlement of the polychaete Hydroides elegans. It has been suggested that IBMX triggers larval settlement by elevating the cellular adenosine 3 ',5 '-cyclic monophosphate (CAMP) level in this species. To test this hypothesis, we first examined cAMP-level changes in both the competent (CL) and attached larvae (AL) and then characterized the cAMP-dependent protein kinase in H. elegans, which is the major mediator of cAMP action. Tissue extracts of the larvae were assayed for cAMP by enzyme immunoassay; the results showed that IBMX increased cAMP production up to approximately two-folds in the CL. However, there was no significant difference in the cAMP concentration between the CL and AL that were not treated with IBMX. The catalytic subunit of protein kinase A gene from H. elegans (designated HePKAc) was cloned, and its expression in different developmental stages of H. elegans was examined using quantitative real-time polymerase chain reaction. The gene expression level in the pre-competent trochophore larvae was the lowest, increased in the CL, reached the highest in the larvae undergoing normal and IBMX-induced metamorphosis, and then decreased in the adult stage. In situ hybridization results showed that HePKAC expressed mainly around eye regions and along body fragments of the CL and AL. Our results indicated that the IBMX-induced cAMP changes and the cAMP-dependent protein kinase gene may mediate larval development and settlement of H. elegans. J. Exp. Zool. (Mol. Dev. Evol.) 31013:417-427, 2008. (C) 2008 Wiley-Liss, Inc. |
| DOI | 10.1002/jez.b.21214 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000257335500003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32610 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Hong Kong Univ Sci & Technol, Atmospher Marine & Coastal Environm Program, Dept Biol, Kowloon, Hong Kong, Peoples R China 2.Hong Kong Univ Sci & Technol, Coastal Marine Lab, Dept Biol, Kowloon, Hong Kong, Peoples R China 3.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Hong-Lei,Song, Lin-Sheng,Qian, Pei-Yuan. Cyclic AMP concentration and protein kinase a (PKA) gene expression at different developmental stages of the polychaete Hydroides elegans[J]. JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION,2008,310B(5):417-427. |
| APA | Li, Hong-Lei,Song, Lin-Sheng,&Qian, Pei-Yuan.(2008).Cyclic AMP concentration and protein kinase a (PKA) gene expression at different developmental stages of the polychaete Hydroides elegans.JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION,310B(5),417-427. |
| MLA | Li, Hong-Lei,et al."Cyclic AMP concentration and protein kinase a (PKA) gene expression at different developmental stages of the polychaete Hydroides elegans".JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION 310B.5(2008):417-427. |
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