Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Molecular cloning and characterization of phytoene synthase gene from a unicellular green alga Haematococcus pluvialis | |
| Liang Cheng-Wei; Zhao Fang-Qing; Qin Song; Tan Cong-Ping; Wei Wei; Meng Chun-Xiao | |
| 2006-09-01 | |
| 发表期刊 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
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| 卷号 | 33期号:9页码:854-860 |
| 文章类型 | Article |
| 摘要 | The unicellular green alga Haematococcus pluvialis accumulates a highly valuable ketocarotenoid, i.e. astaxanthin up to 4% dry weight under stress conditions. Phytoene synthase is considered to be the first rate limiting enzyme in carotenoid biosynthesis pathway in H. pluvialis. The cDNA and genomic genes of phytoene synthase, i.e. psy from H.pluvialis were cloned and characterized. Result showed that psy had one open reading frame of 1 200 bp encoding a putative polypeptide of 400 amino acids which was interrupted by four introns. Phylogenetic analysis revealed that psy from green algae formed a monophyletic clade, and its closer relationship was higher plants. By using genomic walking approach, an approximate 1 kb 5' flanking region of psy gene was cloned and a number of putative cis-regulatory elements were revealed. Fusing a 297 bp internal sequence (-297 to -1 bp from the translation initiation codon of psy) with the reporter gene, i.e. lacZ before attempted introducing the construct into the green alga via particle bombardment resulted in lacZ transient expression. |
| 关键词 | Haematococcus Pluvialis Phytoene Synthase 5 ' Flanking Region Phylogenetic Analysis Promoter |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000240833200008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/32541 |
| 专题 | 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Grad Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang Cheng-Wei,Zhao Fang-Qing,Qin Song,et al. Molecular cloning and characterization of phytoene synthase gene from a unicellular green alga Haematococcus pluvialis[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2006,33(9):854-860. |
| APA | Liang Cheng-Wei,Zhao Fang-Qing,Qin Song,Tan Cong-Ping,Wei Wei,&Meng Chun-Xiao.(2006).Molecular cloning and characterization of phytoene synthase gene from a unicellular green alga Haematococcus pluvialis.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,33(9),854-860. |
| MLA | Liang Cheng-Wei,et al."Molecular cloning and characterization of phytoene synthase gene from a unicellular green alga Haematococcus pluvialis".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 33.9(2006):854-860. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Molecular cloning an（580KB） | 期刊论文 | 出版稿 | 限制开放 | ODC PDDL | 浏览 | |
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