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The microsatellites and minisatellites in the genome of Fenneropenaeus chinensis
Gao, H; Kong, J
2005-12-01
发表期刊DNA SEQUENCE
卷号16期号:6页码:426-436
文章类型Article
摘要Through two-time sequencing randomly in Fenneropenaeus chinensis , 2,597,000 bp cumulative length random genomic sequences about occupying 1.23% of the entire genome are obtained, in which the length of the first time sequencing is 8,84,000 bp, by cutting the genome DNA with Sau3AI enzyme, and the second is 1,713,000 bp by breaking the genome DNA with the physical method, ultrasonic. Using tandem repeat finder (TRF) soft to analyze the sequences, 4588 tandem repeats are found, in which the number of microsatellites (1-6 bp) is 3888, and 700 for minisatellites (>= 7 bp). The cumulative length of repeats is 3,05,555 bp, accounting for 11.72% of total cumulative sequence length, in which the cumulative length of microsatellites is 2,32,979 bp, accounting for 8.97% of total sequence length, and greater than those of other organisms, such as human and mosquito, etc. The dinucleotide repeat type is dominant in which the dominant repeat class is AT. The second abundant repeat type is trinucleotide, of which the dominant repeat class is AAT. Interestingly, of all of repeat types, the repeat numbers and repeat classes of primer number repeat types, such as pentanucleotide, heptanucleotide, elevennucleotide, etc. are less than those of repeat types beside them. The phenomena may involve the genesis and the evolution of microsatellites and minisatellites.
关键词Microsatellites Minisatellites F. Chinensis Genome Repeat Types Repeat Classes
DOI10.1080/10425170500354359
收录类别SCI
语种英语
WOS记录号WOS:000233305400004
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/32523
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Gao, H,Kong, J. The microsatellites and minisatellites in the genome of Fenneropenaeus chinensis[J]. DNA SEQUENCE,2005,16(6):426-436.
APA Gao, H,&Kong, J.(2005).The microsatellites and minisatellites in the genome of Fenneropenaeus chinensis.DNA SEQUENCE,16(6),426-436.
MLA Gao, H,et al."The microsatellites and minisatellites in the genome of Fenneropenaeus chinensis".DNA SEQUENCE 16.6(2005):426-436.
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