DNA fingerprinting of selected Laminaria (Phaeophyta) gametophytes by RAPD markers

doi:10.1016/j.aquaculture.2004.05.007

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	DNA fingerprinting of selected Laminaria (Phaeophyta) gametophytes by RAPD markers
	Wang, XL; Yang, YX ; Cong, YZ ; Duan, DL
	2004-09-01
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	238 期号:1-4 页码:143-153
文章类型	Article
摘要	Random amplified polymorphism DNA (RAPD) analysis was applied to germplasm characterization in 33 different selected Laminaria male and female gametophytes. The positional homology of the RAPD analysis using sequence characterized applied region (SCAR) method was successfully conducted. A total of 233 polymorphic loci were obtained from 18 selected primers after screening, of which 27 stable and clear bands were selected to construct a fingerprint map for discrimination of each gametophyte. Seven RAPD markers from five primers were finally determined by a computer program to construct the fingerprint map. Three specific markers closely related with gametophytes were obtained and were converted to gametophytic SCAR markers, the first SCAR marker report on Laminaria germplasm and applicable to cultivars identification. These results demonstrated the feasibility of applying RAPD markers to germplasm characterization in selected Laminaria gametophytes, and can provide a molecular basis for breeding new Laminaria strains. (C) 2004 Elsevier B.V. All rights reserved.; Random amplified polymorphism DNA (RAPD) analysis was applied to germplasm characterization in 33 different selected Laminaria male and female gametophytes. The positional homology of the RAPD analysis using sequence characterized applied region (SCAR) method was successfully conducted. A total of 233 polymorphic loci were obtained from 18 selected primers after screening, of which 27 stable and clear bands were selected to construct a fingerprint map for discrimination of each gametophyte. Seven RAPD markers from five primers were finally determined by a computer program to construct the fingerprint map. Three specific markers closely related with gametophytes were obtained and were converted to gametophytic SCAR markers, the first SCAR marker report on Laminaria germplasm and applicable to cultivars identification. These results demonstrated the feasibility of applying RAPD markers to germplasm characterization in selected Laminaria gametophytes, and can provide a molecular basis for breeding new Laminaria strains. (C) 2004 Elsevier B.V. All rights reserved.
关键词	Laminaria Gametophytes Germplasm Rapd Scar Fingerprint
学科领域	Fisheries ; Marine & Freshwater Biology
DOI	10.1016/j.aquaculture.2004.05.007
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000224004300012
引用统计	被引频次：43[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3228
专题	实验海洋生物学重点实验室海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100081, Peoples R China 3.Shandong Oriental Ocean Sci Tech Co Ltd, Yantai 265618, Peoples R China
推荐引用方式 GB/T 7714	Wang, XL,Yang, YX,Cong, YZ,et al. DNA fingerprinting of selected Laminaria (Phaeophyta) gametophytes by RAPD markers[J]. AQUACULTURE,2004,238(1-4):143-153.
APA	Wang, XL,Yang, YX,Cong, YZ,&Duan, DL.(2004).DNA fingerprinting of selected Laminaria (Phaeophyta) gametophytes by RAPD markers.AQUACULTURE,238(1-4),143-153.
MLA	Wang, XL,et al."DNA fingerprinting of selected Laminaria (Phaeophyta) gametophytes by RAPD markers".AQUACULTURE 238.1-4(2004):143-153.

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