Product monitoring and quantitation of oligosaccharides composition in agar hydrolysates by precolumn labeling HPLC

doi:10.1016/j.talanta.2004.04.002

IOCAS-IR > 实验海洋生物学重点实验室

	Product monitoring and quantitation of oligosaccharides composition in agar hydrolysates by precolumn labeling HPLC
	Chen, HM ; Zheng, L ; Lin, W ; Yan, XJ
	2004-10-20
发表期刊	TALANTA
ISSN	0039-9140
卷号	64 期号:3 页码:773-777
文章类型	Article
摘要	Since the discovery of multiple bioactivities for agarobiose oligomers, a quantitative method has been in great need to monitor the agarobiose oligomers. This report demonstrates that agarobiose oligomers can be separated with high resolution in HPLC after introducing a-naphthylamine into compounds. Agarobiose oligomers ranged from biose to decaose were isolated by Sephadex column. HPLC analysis indicated that each oliomer could be quantified with good linearity and a low detection limit of 0.1-4 mug/ml. The chromatographic profiles of agaro-oligosaccharides with different hydrolysis modes (hydrochloride, citric acid, solid acid, and hydroxyl radical degradation) showed that agarobiose could be obtained more than 57.8% using solid acid mediated hydrolysis, while hydrochloride acid could degrade agar into a series of agaro-oligosaccharides from biose to decaose. The yield of oligosaccharides was low if hydrolyzed by citric acid. The Fenton degradation can increase the speed of hydrolysis, but the product was complex. (C) 2004 Elsevier B.V. All rights reserved.; Since the discovery of multiple bioactivities for agarobiose oligomers, a quantitative method has been in great need to monitor the agarobiose oligomers. This report demonstrates that agarobiose oligomers can be separated with high resolution in HPLC after introducing a-naphthylamine into compounds. Agarobiose oligomers ranged from biose to decaose were isolated by Sephadex column. HPLC analysis indicated that each oliomer could be quantified with good linearity and a low detection limit of 0.1-4 mug/ml. The chromatographic profiles of agaro-oligosaccharides with different hydrolysis modes (hydrochloride, citric acid, solid acid, and hydroxyl radical degradation) showed that agarobiose could be obtained more than 57.8% using solid acid mediated hydrolysis, while hydrochloride acid could degrade agar into a series of agaro-oligosaccharides from biose to decaose. The yield of oligosaccharides was low if hydrolyzed by citric acid. The Fenton degradation can increase the speed of hydrolysis, but the product was complex. (C) 2004 Elsevier B.V. All rights reserved.
关键词	Agarobiose Oligomers Hplc Alpha-naphthylamine Agaro-oligosaccharides
学科领域	Chemistry, Analytical
DOI	10.1016/j.talanta.2004.04.002
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000224285300029
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3184
专题	实验海洋生物学重点实验室
作者单位	1.Ningbo Univ, Key Lab Marine Biotechnol, Ningbo 315211, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Chen, HM,Zheng, L,Lin, W,et al. Product monitoring and quantitation of oligosaccharides composition in agar hydrolysates by precolumn labeling HPLC[J]. TALANTA,2004,64(3):773-777.
APA	Chen, HM,Zheng, L,Lin, W,&Yan, XJ.(2004).Product monitoring and quantitation of oligosaccharides composition in agar hydrolysates by precolumn labeling HPLC.TALANTA,64(3),773-777.
MLA	Chen, HM,et al."Product monitoring and quantitation of oligosaccharides composition in agar hydrolysates by precolumn labeling HPLC".TALANTA 64.3(2004):773-777.

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