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A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms
Chen, HM; Yan, XJ; Lin, W; Zheng, L; Zhang, WW
2004-09-01
发表期刊PHARMACEUTICAL BIOLOGY
ISSN1388-0209
卷号42期号:6页码:416-421
文章类型Article
摘要A new enzyme assay method for screening alpha-glucosidase inhibitors with rapidity and simplicity was developed. The enzyme-substituted alpha-glucosidases for this assay was glucoamylase. Samples were spotted or developed on the silica gel plate. The agar solution containing substrate was poured on the plate, and paper impregnated with enzyme was layered on the agar. After incubation, an inhibitory circle would appear around the inhibitor. By using this method, more than 200 strains of marine microorganisms were screened. Among them, three active strains were found to secrete inhibitors in the culture medium.; A new enzyme assay method for screening alpha-glucosidase inhibitors with rapidity and simplicity was developed. The enzyme-substituted alpha-glucosidases for this assay was glucoamylase. Samples were spotted or developed on the silica gel plate. The agar solution containing substrate was poured on the plate, and paper impregnated with enzyme was layered on the agar. After incubation, an inhibitory circle would appear around the inhibitor. By using this method, more than 200 strains of marine microorganisms were screened. Among them, three active strains were found to secrete inhibitors in the culture medium.
关键词Enzyme Assay Alpha-glucosidase Inhibitor Marine Microorganism
学科领域Plant Sciences ; Medical Laboratory Technology ; Pharmacology & Pharmacy
DOI10.1080/13880200490885987
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000226444900006
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/3178
专题实验海洋生物学重点实验室
作者单位1.Ningbo Univ, Key Lab Marine Biotechnol, Ningbo 315211, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
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Chen, HM,Yan, XJ,Lin, W,et al. A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms[J]. PHARMACEUTICAL BIOLOGY,2004,42(6):416-421.
APA Chen, HM,Yan, XJ,Lin, W,Zheng, L,&Zhang, WW.(2004).A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms.PHARMACEUTICAL BIOLOGY,42(6),416-421.
MLA Chen, HM,et al."A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms".PHARMACEUTICAL BIOLOGY 42.6(2004):416-421.
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