Toxicity and protective efficiency of cryoprotectants to flounder (Paralichthys olivaceus) embryos

doi:10.1016/j.theriogenology.2004.04.011

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	Toxicity and protective efficiency of cryoprotectants to flounder (Paralichthys olivaceus) embryos
	Zhang, YZ ; Zhang, SC ; Liu, XZ ; Xu, YJ; Hu, JH ; Xu, YY ; Li, J; Chen, SL
	2005-02-01
发表期刊	THERIOGENOLOGY
ISSN	0093-691X
卷号	63 期号:3 页码:763-773
文章类型	Article
摘要	With the purpose of finding an ideal cryoprotectant or combination of cryoprotectants in a suitable concentration for flounder (Paralichthys olivaceus) embryo cryopreservation, we tested the toxicities, at culture temperature (16 degrees C), of five most commonly used cryoprotectants-dimethyl sulfoxide (Me2SO), glycerol, methanol (MeOH), 1,2-propylene glycol (PG) and ethylene glycol (EG). In addition, cryoprotective efficiency to flounder embryos of individual and combined cryoprotectants were tested at -15 degrees C for 60 min. Five different concentrations of each of the five cryoprotectants and 20 different combinations of these cryoprotectants were tested for their protective efficiency. The results showed that the toxicity to flounder embryos of the five cryoprotectants are in the following sequence: PG < MeOH < Me2SO < glycerol < EG (P < 0.05); whereas the protective efficiency of each cryoprotectant, at -15 degrees C for a period of 60 min, are in the following sequence: PG > Me2SO approximate to MeOH approximate to glycerol > EG (greater symbols mean P < 0.05, and approximate symbols mean P > 0.05). Methanol combined with any one of the other cryoprotectants gave the best protection, while ethylene glycol combined with any one of the other cryoprotectants gave the poorest protection at -15 degrees C. Toxicity effect was concentration dependent with the lowest concentration being the least toxic for all five cryoprotectants at 16 degrees C. For PG, MeOH and glycerol, 20% solutions gave the best protection at -15 degrees C; whereas a 15% solution of Me2SO, and a 10% solution of EG, gave the best protection at -15 degrees C. (c) 2004 Elsevier Inc. All rights reserved.; With the purpose of finding an ideal cryoprotectant or combination of cryoprotectants in a suitable concentration for flounder (Paralichthys olivaceus) embryo cryopreservation, we tested the toxicities, at culture temperature (16 degrees C), of five most commonly used cryoprotectants-dimethyl sulfoxide (Me2SO), glycerol, methanol (MeOH), 1,2-propylene glycol (PG) and ethylene glycol (EG). In addition, cryoprotective efficiency to flounder embryos of individual and combined cryoprotectants were tested at -15 degrees C for 60 min. Five different concentrations of each of the five cryoprotectants and 20 different combinations of these cryoprotectants were tested for their protective efficiency. The results showed that the toxicity to flounder embryos of the five cryoprotectants are in the following sequence: PG < MeOH < Me2SO < glycerol < EG (P < 0.05); whereas the protective efficiency of each cryoprotectant, at -15 degrees C for a period of 60 min, are in the following sequence: PG > Me2SO approximate to MeOH approximate to glycerol > EG (greater symbols mean P < 0.05, and approximate symbols mean P > 0.05). Methanol combined with any one of the other cryoprotectants gave the best protection, while ethylene glycol combined with any one of the other cryoprotectants gave the poorest protection at -15 degrees C. Toxicity effect was concentration dependent with the lowest concentration being the least toxic for all five cryoprotectants at 16 degrees C. For PG, MeOH and glycerol, 20% solutions gave the best protection at -15 degrees C; whereas a 15% solution of Me2SO, and a 10% solution of EG, gave the best protection at -15 degrees C. (c) 2004 Elsevier Inc. All rights reserved.
关键词	Flounder Embryo Cryoprotectant Toxicity Protective Efficiency
学科领域	Reproductive Biology ; Veterinary Sciences
DOI	10.1016/j.theriogenology.2004.04.011
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000227660400007
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3168
专题	实验海洋生物学重点实验室
作者单位	1.Ocean Univ Qingdao, Dept Marine Biol, Qingdao 266003, Peoples R China 2.Liaocheng Univ, Dept Life Sci, Liaocheng 252059, Peoples R China 3.Chinese Acad Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang, YZ,Zhang, SC,Liu, XZ,et al. Toxicity and protective efficiency of cryoprotectants to flounder (Paralichthys olivaceus) embryos[J]. THERIOGENOLOGY,2005,63(3):763-773.
APA	Zhang, YZ.,Zhang, SC.,Liu, XZ.,Xu, YJ.,Hu, JH.,...&Chen, SL.(2005).Toxicity and protective efficiency of cryoprotectants to flounder (Paralichthys olivaceus) embryos.THERIOGENOLOGY,63(3),763-773.
MLA	Zhang, YZ,et al."Toxicity and protective efficiency of cryoprotectants to flounder (Paralichthys olivaceus) embryos".THERIOGENOLOGY 63.3(2005):763-773.

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