Identification of 27 Porphyra lines (Rhodophyta) by DNA fingerprinting and molecular markers

doi:10.1007/s10811-005-4845-8

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	Identification of 27 Porphyra lines (Rhodophyta) by DNA fingerprinting and molecular markers
	Weng, ML ; Liu, B ; Jin, DM ; Yang, QK ; Zhao, G ; Ma, JH ; Xu, P; Duan, DL ; Wang, B
	2005
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	17 期号:1 页码:91-97
文章类型	Article
摘要	Twenty-seven Porphyra lines, including lines widely used in China, wild lines and lines introduced to China from abroad in recent years, were screened by random amplified polymorphic DNA (RAPD) technique with 120 operon primers. From the generated RAPD products, 11 bands that showed stable and repeatable RAPD patterns amplified by OPC-04, OPJ-18 and OPX-06, respectively were scored and used to develop the DNA fingerprints of the 27 Porphyra lines. Moreover, the DNA fingerprinting patterns were converted into computer language expressed with two digitals, 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of the corresponding band, respectively. Based on the above results, computerized DNA fingerprints were constructed in which each of the 27 Porphyra lines has its unique fingerprinting pattern and can be easily distinguished from others. Software named PGI (Porphyra germplasm identification) was designed for identification of the 27 Porphyra lines. In addition, seven specific RAPD markers from seven Porphyra lines were identified and two of them were successfully converted into SCAR (sequence characterized amplification region) markers. The developed DNA fingerprinting and specific molecular markers provide useful ways for the identification, classification and resource protection of the Porphyra lines.; Twenty-seven Porphyra lines, including lines widely used in China, wild lines and lines introduced to China from abroad in recent years, were screened by random amplified polymorphic DNA (RAPD) technique with 120 operon primers. From the generated RAPD products, 11 bands that showed stable and repeatable RAPD patterns amplified by OPC-04, OPJ-18 and OPX-06, respectively were scored and used to develop the DNA fingerprints of the 27 Porphyra lines. Moreover, the DNA fingerprinting patterns were converted into computer language expressed with two digitals, 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of the corresponding band, respectively. Based on the above results, computerized DNA fingerprints were constructed in which each of the 27 Porphyra lines has its unique fingerprinting pattern and can be easily distinguished from others. Software named PGI (Porphyra germplasm identification) was designed for identification of the 27 Porphyra lines. In addition, seven specific RAPD markers from seven Porphyra lines were identified and two of them were successfully converted into SCAR (sequence characterized amplification region) markers. The developed DNA fingerprinting and specific molecular markers provide useful ways for the identification, classification and resource protection of the Porphyra lines.
关键词	Dna Fingerprinting Porphyra Random Amplified Polymorphic Dna (Rapd) Sequence Characterized Amplification Region (Scar) Sequence Tagged Site (Sts) Specific Molecular Markers
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI	10.1007/s10811-005-4845-8
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000229904700014
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3142
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Shanghai Fisheries Univ, Shanghai 200090, Peoples R China 3.Jiangsu Inst Oceanol & Hydrol, Nantong 226007, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Weng, ML,Liu, B,Jin, DM,et al. Identification of 27 Porphyra lines (Rhodophyta) by DNA fingerprinting and molecular markers[J]. JOURNAL OF APPLIED PHYCOLOGY,2005,17(1):91-97.
APA	Weng, ML.,Liu, B.,Jin, DM.,Yang, QK.,Zhao, G.,...&Wang, B.(2005).Identification of 27 Porphyra lines (Rhodophyta) by DNA fingerprinting and molecular markers.JOURNAL OF APPLIED PHYCOLOGY,17(1),91-97.
MLA	Weng, ML,et al."Identification of 27 Porphyra lines (Rhodophyta) by DNA fingerprinting and molecular markers".JOURNAL OF APPLIED PHYCOLOGY 17.1(2005):91-97.

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