Isolation of fucoxanthin from the rhizoid of Laminaria japonica Aresch

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	Isolation of fucoxanthin from the rhizoid of Laminaria japonica Aresch
	Wang, WJ ; Wang, GC ; Zhang, M; Tseng, CK
	2005-08-01
发表期刊	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
ISSN	1672-9072
卷号	47 期号:8 页码:1009-1015
文章类型	Article
摘要	Fucoxanthin was extracted from the intact rhizoid of Laminaria japonica Aresch with dimethyl sulfoxide (DMSO), and then recovered from the DMSO extract by partitioning into ethyl acetate and subsequent evaporation. Some isolation conditions such as solvent volume and extraction time were screened. The quantity and quality of the extracted fucoxanthin were determined by spectral analysis (absorption spectra and fluorescence emission spectra). The results indicated that: (1) the average total content of fucoxanthin was 122.1 mu g in 1 g of fresh L japonica rhizoid; (2) in comparison with the widely used organic solvent, acetone, DMSO was much more effective for the extraction of fucoxanthin; (3) both DMSO volume and extraction time influenced extraction efficiency such as the recovery rate and purity of fucoxanthin (1 g of fresh L. japonica rhizoid treated with 4 mL DMSO for 60 min, yielded > 88% of the total fucoxanthin with purity 0.63); (4) when (NH4)(2)SO4 concentration was in the range of 0.5- 1.0 mol/L, the pigments rapidly and entirely moved from DMSO into the ethyl acetate phase; (5) the ethyl acetate and DMSO were recycled using a rotary evaporator.; Fucoxanthin was extracted from the intact rhizoid of Laminaria japonica Aresch with dimethyl sulfoxide (DMSO), and then recovered from the DMSO extract by partitioning into ethyl acetate and subsequent evaporation. Some isolation conditions such as solvent volume and extraction time were screened. The quantity and quality of the extracted fucoxanthin were determined by spectral analysis (absorption spectra and fluorescence emission spectra). The results indicated that: (1) the average total content of fucoxanthin was 122.1 mu g in 1 g of fresh L japonica rhizoid; (2) in comparison with the widely used organic solvent, acetone, DMSO was much more effective for the extraction of fucoxanthin; (3) both DMSO volume and extraction time influenced extraction efficiency such as the recovery rate and purity of fucoxanthin (1 g of fresh L. japonica rhizoid treated with 4 mL DMSO for 60 min, yielded > 88% of the total fucoxanthin with purity 0.63); (4) when (NH4)(2)SO4 concentration was in the range of 0.5- 1.0 mol/L, the pigments rapidly and entirely moved from DMSO into the ethyl acetate phase; (5) the ethyl acetate and DMSO were recycled using a rotary evaporator.
关键词	Dimethyl Sulfoxide Extraction Fucoxanthin Laminaria Japonica Rhizoid
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000230928500013
引用统计	被引频次：48[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3138
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Bur Environm Protect Feixi Cty Anhui Prov, Hefei 231200, Peoples R China
推荐引用方式 GB/T 7714	Wang, WJ,Wang, GC,Zhang, M,et al. Isolation of fucoxanthin from the rhizoid of Laminaria japonica Aresch[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2005,47(8):1009-1015.
APA	Wang, WJ,Wang, GC,Zhang, M,&Tseng, CK.(2005).Isolation of fucoxanthin from the rhizoid of Laminaria japonica Aresch.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,47(8),1009-1015.
MLA	Wang, WJ,et al."Isolation of fucoxanthin from the rhizoid of Laminaria japonica Aresch".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 47.8(2005):1009-1015.

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