Identification of Porphyra lines (Rhodophyta) by AFLP DNA fingerprinting and molecular markers

IOCAS-IR > 实验海洋生物学重点实验室

	Identification of Porphyra lines (Rhodophyta) by AFLP DNA fingerprinting and molecular markers
	Sun, JW ; Jin, DM ; Zhou, CJ ; Yang, QK ; Weng, ML ; Duan, DL ; Xu, P; Ma, JH ; Wang, B
	2005-09-01
发表期刊	PLANT MOLECULAR BIOLOGY REPORTER
ISSN	0735-9640
卷号	23 期号:3 页码:251-262
文章类型	Article
摘要	Twenty-seven Porphyra lines from 5 classes, including lines widely used in China, wild lines, and lines introduced to China from abroad in recent years, were screened by means of amplified fragment length polymorphism (AFLP) with 24 primer pairs. From the generated AFLP products, 13 bands that showed stable and repeatable AFLP patterns amplified by primer pairs M-CGA/E-AA and M-CGA/E-TA were scored and used to develop the DNA fingerprints of the 27 Porphyra lines. Moreover, the DNA fingerprinting patterns were converted into computer language expressed with digitals 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of the corresponding band. On the basis of these results, computerized AFLP DNA fingerprints were constructed in which each of the 27 Porphyra lines has its unique AFLP,fingerprinting pattern and can be easily distinguished from others. Software called PGI-AFLP (Porphyra germplasm identification-AFLP) was designed for identification of the 27 Porphyra lines. In addition, 21 specific AFLP markers from 15 Porphyra lines were identified; 6 AFLP markers from 4 Porphyra lines were sequenced, and 2 of them were successfully converted into SCAR (sequence characterized amplification region) markers. The developed AFLP DNA fingerprinting and specific molecular markers provide useful ways for the identification, classification, and resource protection of the Porphyra lines.; Twenty-seven Porphyra lines from 5 classes, including lines widely used in China, wild lines, and lines introduced to China from abroad in recent years, were screened by means of amplified fragment length polymorphism (AFLP) with 24 primer pairs. From the generated AFLP products, 13 bands that showed stable and repeatable AFLP patterns amplified by primer pairs M-CGA/E-AA and M-CGA/E-TA were scored and used to develop the DNA fingerprints of the 27 Porphyra lines. Moreover, the DNA fingerprinting patterns were converted into computer language expressed with digitals 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of the corresponding band. On the basis of these results, computerized AFLP DNA fingerprints were constructed in which each of the 27 Porphyra lines has its unique AFLP,fingerprinting pattern and can be easily distinguished from others. Software called PGI-AFLP (Porphyra germplasm identification-AFLP) was designed for identification of the 27 Porphyra lines. In addition, 21 specific AFLP markers from 15 Porphyra lines were identified; 6 AFLP markers from 4 Porphyra lines were sequenced, and 2 of them were successfully converted into SCAR (sequence characterized amplification region) markers. The developed AFLP DNA fingerprinting and specific molecular markers provide useful ways for the identification, classification, and resource protection of the Porphyra lines.
关键词	Amplified Fragment Length Polymorphism Dna Fingerprinting Porphyra Line Sequence Characterized Amplified Region Sequence Tagged Site (Sts) Specific Molecular Marker
学科领域	Biochemical Research Methods ; Plant Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000235577400005
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3126
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Laiyang Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Jiangsu Inst Oceanol & Hydrol, Nantong 226007, Peoples R China 5.Shanghai Fisheries Univ, Shanghai 200090, Peoples R China
推荐引用方式 GB/T 7714	Sun, JW,Jin, DM,Zhou, CJ,et al. Identification of Porphyra lines (Rhodophyta) by AFLP DNA fingerprinting and molecular markers[J]. PLANT MOLECULAR BIOLOGY REPORTER,2005,23(3):251-262.
APA	Sun, JW.,Jin, DM.,Zhou, CJ.,Yang, QK.,Weng, ML.,...&Wang, B.(2005).Identification of Porphyra lines (Rhodophyta) by AFLP DNA fingerprinting and molecular markers.PLANT MOLECULAR BIOLOGY REPORTER,23(3),251-262.
MLA	Sun, JW,et al."Identification of Porphyra lines (Rhodophyta) by AFLP DNA fingerprinting and molecular markers".PLANT MOLECULAR BIOLOGY REPORTER 23.3(2005):251-262.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
sun-Identification o（367KB）			限制开放	--	浏览