Cloning and characterization of beta-carotene ketolase gene promoter in Haematococcus pluvialis

doi:10.1111/j.1745-7270.2005.00033.x

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	Cloning and characterization of beta-carotene ketolase gene promoter in Haematococcus pluvialis
	Meng, CX ; Teng, CY ; Jiang, P ; Qin, S ; Tseng, CK
	2005-04-01
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN	1672-9145
卷号	37 期号:4 页码:270-275
文章类型	Article
摘要	The unicellular green alga Haematococcus pluvialis accumulates a highly valuable ketocarotenoid, astaxanthin, under various environmental stresses. beta-carotene ketolase (BKT) plays a key role in astaxanthin biosynthesis in H. pluvialis. In this paper, an approximate 700 bp 5'-flanking region of the bkt gene containing a putative promoter was cloned through walking upstream. The results of the sequence analysis showed that this bkt 5'-flanking region might have cis-acting elements such as sterol regulatory element (SRE-1)-like motifs, the C-repeat/dehydration responsive element (DRE) and al-3 proximal element (APE)-like motifs, except for typical TATA and CCAAT boxes. The results of the P-galactosidase assay and the transient expression of lacZ driven by a series of sequential deletions revealed that a minimal promoter-like region might exist from -630 to -408 bp, and the highest promoter activity was observed to span the positions from -630 to -308 bp. The results of the site-directed mutagenesis of a C-repeat/DRE and two APE-like motifs in a promoter-like region (-630 to -308 bp) suggested that two APE-like motifs might be essential for transcriptional control of the bkt gene.; The unicellular green alga Haematococcus pluvialis accumulates a highly valuable ketocarotenoid, astaxanthin, under various environmental stresses. beta-carotene ketolase (BKT) plays a key role in astaxanthin biosynthesis in H. pluvialis. In this paper, an approximate 700 bp 5'-flanking region of the bkt gene containing a putative promoter was cloned through walking upstream. The results of the sequence analysis showed that this bkt 5'-flanking region might have cis-acting elements such as sterol regulatory element (SRE-1)-like motifs, the C-repeat/dehydration responsive element (DRE) and al-3 proximal element (APE)-like motifs, except for typical TATA and CCAAT boxes. The results of the P-galactosidase assay and the transient expression of lacZ driven by a series of sequential deletions revealed that a minimal promoter-like region might exist from -630 to -408 bp, and the highest promoter activity was observed to span the positions from -630 to -308 bp. The results of the site-directed mutagenesis of a C-repeat/DRE and two APE-like motifs in a promoter-like region (-630 to -308 bp) suggested that two APE-like motifs might be essential for transcriptional control of the bkt gene.
关键词	Haematococcus Pluvialis Astaxanthin Beta-carotene Ketolase Gene (Bkt) Cis-acting Element Promoter
学科领域	Biochemistry & Molecular Biology ; Biophysics
DOI	10.1111/j.1745-7270.2005.00033.x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000228545300009
引用统计	被引频次：28[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3112
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Meng, CX,Teng, CY,Jiang, P,et al. Cloning and characterization of beta-carotene ketolase gene promoter in Haematococcus pluvialis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(4):270-275.
APA	Meng, CX,Teng, CY,Jiang, P,Qin, S,&Tseng, CK.(2005).Cloning and characterization of beta-carotene ketolase gene promoter in Haematococcus pluvialis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(4),270-275.
MLA	Meng, CX,et al."Cloning and characterization of beta-carotene ketolase gene promoter in Haematococcus pluvialis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.4(2005):270-275.

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