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Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli
Ge, BS; Tang, ZH; Lin, LP; Ren, YH; Yang, Y; Qin, S
2005-06-01
发表期刊BIOTECHNOLOGY LETTERS
ISSN0141-5492
卷号27期号:11页码:783-787
文章类型Article
摘要A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amylose affinity column. The purity of product was greater than 98% and yielded an average of 5.5 g kg(-1) dry cells. Recombinant allophycocyanin significantly inhibited H-22 hepatoma (p (0.01) in mice with inhibition rates ranging from 36% to 62% with doses from 6.25 to 50 mg kg(-1) d(-1).; A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amylose affinity column. The purity of product was greater than 98% and yielded an average of 5.5 g kg(-1) dry cells. Recombinant allophycocyanin significantly inhibited H-22 hepatoma (p (0.01) in mice with inhibition rates ranging from 36% to 62% with doses from 6.25 to 50 mg kg(-1) d(-1).
关键词Allophycocyanin Escherichia Coli Protein Purification Recombinant Allophycocyanin Tumor Inhibition
学科领域Biotechnology & Applied Microbiology
DOI10.1007/s10529-005-5794-7
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收录类别SCI
语种英语
WOS记录号WOS:000231065100008
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被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/3098
专题实验海洋生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Inst Mat Med, Shanghai 201203, Peoples R China
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Ge, BS,Tang, ZH,Lin, LP,et al. Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli[J]. BIOTECHNOLOGY LETTERS,2005,27(11):783-787.
APA Ge, BS,Tang, ZH,Lin, LP,Ren, YH,Yang, Y,&Qin, S.(2005).Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli.BIOTECHNOLOGY LETTERS,27(11),783-787.
MLA Ge, BS,et al."Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli".BIOTECHNOLOGY LETTERS 27.11(2005):783-787.
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