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Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli | |
Ge, BS; Tang, ZH; Lin, LP; Ren, YH; Yang, Y; Qin, S | |
2005-06-01 | |
发表期刊 | BIOTECHNOLOGY LETTERS |
ISSN | 0141-5492 |
卷号 | 27期号:11页码:783-787 |
文章类型 | Article |
摘要 | A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amylose affinity column. The purity of product was greater than 98% and yielded an average of 5.5 g kg(-1) dry cells. Recombinant allophycocyanin significantly inhibited H-22 hepatoma (p (0.01) in mice with inhibition rates ranging from 36% to 62% with doses from 6.25 to 50 mg kg(-1) d(-1).; A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amylose affinity column. The purity of product was greater than 98% and yielded an average of 5.5 g kg(-1) dry cells. Recombinant allophycocyanin significantly inhibited H-22 hepatoma (p (0.01) in mice with inhibition rates ranging from 36% to 62% with doses from 6.25 to 50 mg kg(-1) d(-1). |
关键词 | Allophycocyanin Escherichia Coli Protein Purification Recombinant Allophycocyanin Tumor Inhibition |
学科领域 | Biotechnology & Applied Microbiology |
DOI | 10.1007/s10529-005-5794-7 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000231065100008 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/3098 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Inst Mat Med, Shanghai 201203, Peoples R China |
推荐引用方式 GB/T 7714 | Ge, BS,Tang, ZH,Lin, LP,et al. Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli[J]. BIOTECHNOLOGY LETTERS,2005,27(11):783-787. |
APA | Ge, BS,Tang, ZH,Lin, LP,Ren, YH,Yang, Y,&Qin, S.(2005).Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli.BIOTECHNOLOGY LETTERS,27(11),783-787. |
MLA | Ge, BS,et al."Pilot-scale fermentation and purification of the recombinant allophycocyanin over-expressed in Escherichia coli".BIOTECHNOLOGY LETTERS 27.11(2005):783-787. |
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