Cloning and characterization of a novel C-type lectin gene from shrimp Litopenaeus vannamei

doi:10.1016/j.fsi.2008.03.008

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	Cloning and characterization of a novel C-type lectin gene from shrimp Litopenaeus vannamei
	Zhang, Ying 1,2; Qiu, Limei 1; Song, Linsheng 1; Zhang, Huan 1,2; Zhao, Jianmin 1; Wang, Lingling 1; Yu, Yundong 1,2; Li, Chenghua 1,2; Li, Fengmei 1,3; Xing, Kezhi 4; Huang, Bingxin 1
	2009
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	26 期号:1 页码:183-192
文章类型	Article
摘要	In invertebrates, C-type lectins play crucial roles in innate immunity responses by mediating the recognition of host cells to pathogens and clearing microinvaders, which interact with carbohydrates and function as pattern recognition receptors (PRRs). A novel C-type lectin gene (LvLec) cDNA was cloned from hemocytes of Litopenaeus vannamei by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of LvLec was of 618 bp, consisting of a 5'-terminal untranslated region (UTR) of 60 bp and a 3'-UTR of 87 bp with a poly (A) tail. The deduced amino acid sequence of LvLec possessed all conserved features critical for the fundamental structure, such as the four cysteine residues (Cys(53), Cys(128), Cys(144), Cys(152)) involved in the formation of disulfides bridges and the potential Ca(2+)/carbohydrate-binding sites. The high similarity and the close phylogenetic relationship of LvLec shared with C-type lectins from vertebrates and invertebrates. The structural features of LvLec indicated that it was an invertebrate counterpart of the C-type lectin family. The cDNA fragment encoding the mature peptide of LvLec was recombined and expressed in Escherichia coli BL21(DE3)-pLysS. The recombinant protein (rLvLec) could agglutinate bacteria E. coli JM109 depending on Ca(2+), and the agglutination could be inhibited by mannose and EDTA. These results indicated that LvLec was a new member of C-type lectin family and involved in the immune defence response to Gram negative bacteria in Litopenaeus vannamei. (C) 2008 Elsevier Ltd. All rights reserved.; In invertebrates, C-type lectins play crucial roles in innate immunity responses by mediating the recognition of host cells to pathogens and clearing microinvaders, which interact with carbohydrates and function as pattern recognition receptors (PRRs). A novel C-type lectin gene (LvLec) cDNA was cloned from hemocytes of Litopenaeus vannamei by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of LvLec was of 618 bp, consisting of a 5'-terminal untranslated region (UTR) of 60 bp and a 3'-UTR of 87 bp with a poly (A) tail. The deduced amino acid sequence of LvLec possessed all conserved features critical for the fundamental structure, such as the four cysteine residues (Cys(53), Cys(128), Cys(144), Cys(152)) involved in the formation of disulfides bridges and the potential Ca2+/carbohydrate-binding sites. The high similarity and the close phylogenetic relationship of LvLec shared with C-type lectins from vertebrates and invertebrates. The structural features of LvLec indicated that it was an invertebrate counterpart of the C-type lectin family. The cDNA fragment encoding the mature peptide of LvLec was recombined and expressed in Escherichia coli BL21(DE3)-pLysS. The recombinant protein (rLvLec) could agglutinate bacteria E. coli JM109 depending on Ca2+, and the agglutination could be inhibited by mannose and EDTA. These results indicated that LvLec was a new member of C-type lectin family and involved in the immune defence response to Gram negative bacteria in Litopenaeus vannamei. (C) 2008 Elsevier Ltd. All rights reserved.
关键词	C-type Lectin Gene Cloning Recombinant Expression Bacteria Agglutination Litopenaeus Vannamei
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2008.03.008
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000264015700023
引用统计	被引频次：75[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3074
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Qingdao Univ Sci & Technol, Qingdao, Peoples R China 4.Tianjin Agr Coll, Tianjin 300381, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Ying,Qiu, Limei,Song, Linsheng,et al. Cloning and characterization of a novel C-type lectin gene from shrimp Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2009,26(1):183-192.
APA	Zhang, Ying.,Qiu, Limei.,Song, Linsheng.,Zhang, Huan.,Zhao, Jianmin.,...&Huang, Bingxin.(2009).Cloning and characterization of a novel C-type lectin gene from shrimp Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,26(1),183-192.
MLA	Zhang, Ying,et al."Cloning and characterization of a novel C-type lectin gene from shrimp Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 26.1(2009):183-192.

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