Cflec-4, a multidomain C-type lectin involved in immune defense of Zhikong scallop Chlamys farreri

doi:10.1016/j.dci.2009.01.003

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	Cflec-4, a multidomain C-type lectin involved in immune defense of Zhikong scallop Chlamys farreri
	Zhang, Huan 1,2; Wang, Hao 1; Wang, Lingling 1; Song, Linsheng 1; Song, Xiaoyan 3; Zhao, Jianmin 1; Li, Ling 1,2; Qiu, Limei 1
	2009
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	33 期号:6 页码:780-788
文章类型	Article
摘要	C-type lectins are a superfamily of carbohydrate-recognition proteins which play crucial roles in the innate immunity. In this study, a novel multidomain C-type lectin gene from scallop Chlamys farreri (designated as Cflec-4) was cloned by RACE approach based on EST analysis. The full-length cDNA of Cflec-4 was of 2086 bp. The open reading frame was of 1830 bp and encoded a polypeptide of 609 amino acids, including a signal sequence and four dissimilar carbohydrate-recognition domains (CRDs). The deduced amino acid sequence of CflecA shared high similarities to other C-type lectin family members. The phylogenetic analysis revealed the divergence between the three N-terminal CRDs and the C-terminal one, suggesting that the four CRDs in Cflec-4 originated by repeated duplication of different primordial CRD. The potential tertiary structure of each CRD in Cflec-4 was typical double-loop structure with Ca(2+)-binding site 2 in the long loop region and two conserved disulfide bridges at the bases of the loops. The tissue distribution of Cflec-4 mRNA was examined by fluorescent quantitative real-time PCR. In the healthy scallops, the Cflec-4 transcripts could be only detected in gonad and hepatopancreas, whereas in the Listonella anguillarum challenged scallops, it could be also detected in hemocytes. These results collectively suggested that CflecA was involved in the immune defense of scallop against pathogen infection and provided new insight into the evolution of C-type lectin superfamily. (C) 2009 Elsevier Ltd. All rights reserved.; C-type lectins are a superfamily of carbohydrate-recognition proteins which play crucial roles in the innate immunity. In this study, a novel multidomain C-type lectin gene from scallop Chlamys farreri (designated as Cflec-4) was cloned by RACE approach based on EST analysis. The full-length cDNA of Cflec-4 was of 2086 bp. The open reading frame was of 1830 bp and encoded a polypeptide of 609 amino acids, including a signal sequence and four dissimilar carbohydrate-recognition domains (CRDs). The deduced amino acid sequence of CflecA shared high similarities to other C-type lectin family members. The phylogenetic analysis revealed the divergence between the three N-terminal CRDs and the C-terminal one, suggesting that the four CRDs in Cflec-4 originated by repeated duplication of different primordial CRD. The potential tertiary structure of each CRD in Cflec-4 was typical double-loop structure with Ca2+-binding site 2 in the long loop region and two conserved disulfide bridges at the bases of the loops. The tissue distribution of Cflec-4 mRNA was examined by fluorescent quantitative real-time PCR. In the healthy scallops, the Cflec-4 transcripts could be only detected in gonad and hepatopancreas, whereas in the Listonella anguillarum challenged scallops, it could be also detected in hemocytes. These results collectively suggested that CflecA was involved in the immune defense of scallop against pathogen infection and provided new insight into the evolution of C-type lectin superfamily. (C) 2009 Elsevier Ltd. All rights reserved.
关键词	Chlamys Farreri C-type Lectin Innate Immunity Gene Cloning Real-time Pcr
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2009.01.003
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000264620700007
引用统计	被引频次：53[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3072
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.NW A&F Univ, Yangling 712100, Shaanxi, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Zhang, Huan,Wang, Hao,Wang, Lingling,et al. Cflec-4, a multidomain C-type lectin involved in immune defense of Zhikong scallop Chlamys farreri[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2009,33(6):780-788.
APA	Zhang, Huan.,Wang, Hao.,Wang, Lingling.,Song, Linsheng.,Song, Xiaoyan.,...&Qiu, Limei.(2009).Cflec-4, a multidomain C-type lectin involved in immune defense of Zhikong scallop Chlamys farreri.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,33(6),780-788.
MLA	Zhang, Huan,et al."Cflec-4, a multidomain C-type lectin involved in immune defense of Zhikong scallop Chlamys farreri".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 33.6(2009):780-788.

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