Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta)

doi:10.1007/s10811-008-9354-0

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	Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta)
	Yao, Jianting 1,2; Fu, Wandong 1,2; Wang, Xiuliang 1; Duan, Delin 1
	2009-04-01
发表期刊	JOURNAL OF APPLIED PHYCOLOGY
ISSN	0921-8971
卷号	21 期号:2 页码:233-238
文章类型	Article
摘要	RNA isolation is difficult in some plants and algae because phenolics, polysaccharides, or other compounds can bind or co-precipitate with RNA, and because the success of RNA isolation can be strain-specific and species-specific. To create an improved RNA isolation protocol for Laminaria japonica Aresch (Laminariaceae, Phaeophyta), four methods for extracting RNA were tested. A cetyltrimethylammonium bromide (CTAB)-based RNA extraction protocol was developed that clearly showed 28S and 18S ribosomal RNA bands and produced RNA with high yield (68 mu g g(-1) fresh weight) and high quality (A (260/280) ratio 1.96 +/- 0.05). The isolated RNA was intact, and RT-PCR analysis confirmed that further molecular application is feasible.; RNA isolation is difficult in some plants and algae because phenolics, polysaccharides, or other compounds can bind or co-precipitate with RNA, and because the success of RNA isolation can be strain-specific and species-specific. To create an improved RNA isolation protocol for Laminaria japonica Aresch (Laminariaceae, Phaeophyta), four methods for extracting RNA were tested. A cetyltrimethylammonium bromide (CTAB)-based RNA extraction protocol was developed that clearly showed 28S and 18S ribosomal RNA bands and produced RNA with high yield (68 mu g g(-1) fresh weight) and high quality (A (260/280) ratio 1.96 +/- 0.05). The isolated RNA was intact, and RT-PCR analysis confirmed that further molecular application is feasible.
关键词	Phaeophyta Phenolics Ctab Polysaccharides Rna Extraction
学科领域	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
DOI	10.1007/s10811-008-9354-0
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000264483600009
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3060
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100059, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yao, Jianting,Fu, Wandong,Wang, Xiuliang,et al. Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta)[J]. JOURNAL OF APPLIED PHYCOLOGY,2009,21(2):233-238.
APA	Yao, Jianting,Fu, Wandong,Wang, Xiuliang,&Duan, Delin.(2009).Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta).JOURNAL OF APPLIED PHYCOLOGY,21(2),233-238.
MLA	Yao, Jianting,et al."Improved RNA isolation from Laminaria japonica Aresch (Laminariaceae, Phaeophyta)".JOURNAL OF APPLIED PHYCOLOGY 21.2(2009):233-238.

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