VEGF gene silencing by cytomegalovirus promoter driven ShRNA expression vector results in vascular development defects in zebrafish

doi:10.1134/S1022795409090038

IOCAS-IR > 实验海洋生物学重点实验室

	VEGF gene silencing by cytomegalovirus promoter driven ShRNA expression vector results in vascular development defects in zebrafish
	Yang, S. L.1; Yan, S.3; Niu, R. L.2; Lin, X. K.2
	2009-09-01
发表期刊	RUSSIAN JOURNAL OF GENETICS
ISSN	1022-7954
卷号	45 期号:9 页码:1040-1046
文章类型	Article
摘要	Zebrafish has been generally considered as an excellent model in case of drug screening, disease model establishment, and vertebrate embryonic development study. In this work, the ability of human cytomegalovirus immediate early promoter (CMV promoter)-driven short hairpin RNA (shRNA) expression vector to induce shRNA against VEGF gene in zebrafish was tested, and its effect on vascular development was assed, too. Using RT-qPCR, blood vessel staining, and in situ hybridization, we confirmed certain transcriptional activity and down regulation of gene expression by the vector. In situ hybridization analysis indicated selective inhibition of NRP1 expression in the VEGF gene loss of function model, which might imply in turn that VEGF could not only activate endothelial cells directly but also could contribute to stimulating angiogenesis in vivo by a mechanism that involved up-regulation of its cognate receptor expression in zebrafish. This contributed to a better understanding of molecular mechanisms of cardiovascular development. The system improved the success rate in making inducible knockdown and widened the possibilities for better therapeutic targets in zebrafish.; Zebrafish has been generally considered as an excellent model in case of drug screening, disease model establishment, and vertebrate embryonic development study. In this work, the ability of human cytomegalovirus immediate early promoter (CMV promoter)-driven short hairpin RNA (shRNA) expression vector to induce shRNA against VEGF gene in zebrafish was tested, and its effect on vascular development was assed, too. Using RT-qPCR, blood vessel staining, and in situ hybridization, we confirmed certain transcriptional activity and down regulation of gene expression by the vector. In situ hybridization analysis indicated selective inhibition of NRP1 expression in the VEGF gene loss of function model, which might imply in turn that VEGF could not only activate endothelial cells directly but also could contribute to stimulating angiogenesis in vivo by a mechanism that involved up-regulation of its cognate receptor expression in zebrafish. This contributed to a better understanding of molecular mechanisms of cardiovascular development. The system improved the success rate in making inducible knockdown and widened the possibilities for better therapeutic targets in zebrafish.
关键词	Endothelial Growth-factor Pol-ii Promoter Rna Interference Angiogenesis Hematopoiesis Neuropilin-1 Mammals System Cell
学科领域	Genetics & Heredity
DOI	10.1134/S1022795409090038
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000269974100003
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3052
专题	实验海洋生物学重点实验室
作者单位	1.CAS, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China 2.CAS, Inst Oceanol, Key Lab Marine Biol, Qingdao 266071, Peoples R China 3.Dalian Jiaotong Univ, Coll Environm & Chem Engn, Dalian 116028, Peoples R China
推荐引用方式 GB/T 7714	Yang, S. L.,Yan, S.,Niu, R. L.,et al. VEGF gene silencing by cytomegalovirus promoter driven ShRNA expression vector results in vascular development defects in zebrafish[J]. RUSSIAN JOURNAL OF GENETICS,2009,45(9):1040-1046.
APA	Yang, S. L.,Yan, S.,Niu, R. L.,&Lin, X. K..(2009).VEGF gene silencing by cytomegalovirus promoter driven ShRNA expression vector results in vascular development defects in zebrafish.RUSSIAN JOURNAL OF GENETICS,45(9),1040-1046.
MLA	Yang, S. L.,et al."VEGF gene silencing by cytomegalovirus promoter driven ShRNA expression vector results in vascular development defects in zebrafish".RUSSIAN JOURNAL OF GENETICS 45.9(2009):1040-1046.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
yang-VEGF gene silen（288KB）			限制开放	--	浏览