Inhibitory effects and mechanisms of high molecular-weight phlorotannins from Sargassum thunbergii on ADP-induced platelet aggregation

doi:10.1007/s00343-009-9166-3

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	Inhibitory effects and mechanisms of high molecular-weight phlorotannins from Sargassum thunbergii on ADP-induced platelet aggregation
	Wei Yuxi 1; Wang Changyun 2; Li Jing3 ; Guo Qi 1; Qi Hongtao 1
	2009-08-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	27 期号:3 页码:558-563
文章类型	Article
摘要	We evaluated the effects of high molecular-weight phlorotannins from Sargassum thunbergii (STP) on ADP-induced platelet aggregation and arachidonic acid (AA) metabolism in New Zealand white rabbits and Wistar rats. The inhibition of STP on platelet aggregation was investigated using a turbidimetric method, and the levels of the terminal products of AA metabolism were measured using the corresponding kits for maleic dialdehyde (MDA), thromboxane B(2) (TXB(2)) and 6-keto-prostaglandin F(1 alpha) (6-keto-PGF(1 alpha)) by colorimetry and radioimmunoassay, as appropriate. We found that STP could inhibit ADP-induced platelet aggregation, and the inhibitory ratio was 91.50% at the STP concentration of 4.0 mg/mL. Furthermore, STP markedly affected AA metabolism by decreasing the synthesis of MDA (P < 0.01) and increasing the synthesis of 6-keto-PGF(1 alpha), thus changing the plasma TXB(2)/6-keto-PGF(1 alpha) balance when the platelets were activated (P < 0.01). Therefore, STP altered AA metabolism and these findings; We evaluated the effects of high molecular-weight phlorotannins from Sargassum thunbergii (STP) on ADP-induced platelet aggregation and arachidonic acid (AA) metabolism in New Zealand white rabbits and Wistar rats. The inhibition of STP on platelet aggregation was investigated using a turbidimetric method, and the levels of the terminal products of AA metabolism were measured using the corresponding kits for maleic dialdehyde (MDA), thromboxane B-2 (TXB2) and 6-keto-prostaglandin F-1 alpha (6-keto-PGF(1 alpha)) by colorimetry and radioimmunoassay, as appropriate. We found that STP could inhibit ADP-induced platelet aggregation, and the inhibitory ratio was 91.50% at the STP concentration of 4.0 mg/mL. Furthermore, STP markedly affected AA metabolism by decreasing the synthesis of MDA (P < 0.01) and increasing the synthesis of 6-keto-PGF(1 alpha), thus changing the plasma TXB2/6-keto-PGF(1 alpha) balance when the platelets were activated (P < 0.01). Therefore, STP altered AA metabolism and these findings
关键词	High Molecular-weight Phlorotannins Sargassum Thunbergii Kuntze Platelet Aggregation Arachidonic Acid Metabolism
学科领域	Limnology ; Oceanography
DOI	10.1007/s00343-009-9166-3
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000270742000019
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3040
专题	实验海洋生物学重点实验室
作者单位	1.Qingdao Univ, Dept Biol, Qingdao 266071, Peoples R China 2.Ocean Univ China, Sch Med & Pharm, Minist Educ, Key Lab Marine Drugs, Qingdao 266003, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wei Yuxi,Wang Changyun,Li Jing,et al. Inhibitory effects and mechanisms of high molecular-weight phlorotannins from Sargassum thunbergii on ADP-induced platelet aggregation[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(3):558-563.
APA	Wei Yuxi,Wang Changyun,Li Jing,Guo Qi,&Qi Hongtao.(2009).Inhibitory effects and mechanisms of high molecular-weight phlorotannins from Sargassum thunbergii on ADP-induced platelet aggregation.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(3),558-563.
MLA	Wei Yuxi,et al."Inhibitory effects and mechanisms of high molecular-weight phlorotannins from Sargassum thunbergii on ADP-induced platelet aggregation".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.3(2009):558-563.

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