Regulation of the Edwardsiella tarda Hemolysin Gene and luxS by EthR

doi:10.4014/jmb.0810.574

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	Regulation of the Edwardsiella tarda Hemolysin Gene and luxS by EthR
	Wang Fang 1,2,3; Zhang, Min 1,2; Hu, Yong-hua 1,2; Zhang, Wei-wei 1,2; Sun, Li 1
	2009-08-01
发表期刊	JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
ISSN	1017-7825
卷号	19 期号:8 页码:765-773
文章类型	Article
摘要	Edwardsiella tarda is a pathogen with a broad host range that includes human and animals. The E. tarda hemolysin (Eth) system, which comprises EthA and EthB, is a noted virulence element that is widely distributed in pathogenic isolates of E. tarda. Previous study has shown that the expression of ethB is regulated by iron, which suggests the possibility that the ferric uptake regulator (Fur) is involved in the regulation of ethB. The work presented in this report supports the previous findings and demonstrates that ethB expression was decreased under conditions when the E. tarda Fur (Fur(Et)) was overproduced, and enhanced when Fur(Et) was inactivated. We also identified a second ethB regulator, EthR, which is a transcription regulator of the GntR family. EthR represses ethB expression by direct interaction with the ethB promoter region. In addition to ethB, EthR also modulates, but positively, luxS expression and AI-2 production by binding to the luxS promoter region. The expression of ethR itself is subject to negative autoregulation; interference with this regulation by overexpressing ethR during the process of infection caused (i) drastic changes in ethB and luxS expressions, (ii) vitiation in the tissue dissemination and survival ability of the bacterium, and (iii) significant attenuation of the overall bacterial virulence. These results not only provide new insights into the regulation mechanisms of the Eth hemolysin and LuxS/AI-2 quorum sensing systems but also highlight the importance of these systems in bacterial virulence.; Edwardsiella tarda is a pathogen with a broad host range that includes human and animals. The E. tarda hemolysin (Eth) system, which comprises EthA and EthB, is a noted virulence element that is widely distributed in pathogenic isolates of E. tarda. Previous study has shown that the expression of ethB is regulated by iron, which suggests the possibility that the ferric uptake regulator (Fur) is involved in the regulation of ethB. The work presented in this report supports the previous findings and demonstrates that ethB expression was decreased under conditions when the E. tarda Fur (Fur(Et)) was overproduced, and enhanced when Fur(Et) was inactivated. We also identified a second ethB regulator, EthR, which is a transcription regulator of the GntR family. EthR represses ethB expression by direct interaction with the ethB promoter region. In addition to ethB, EthR also modulates, but positively, luxS expression and AI-2 production by binding to the luxS promoter region. The expression of ethR itself is subject to negative autoregulation; interference with this regulation by overexpressing ethR during the process of infection caused (i) drastic changes in ethB and luxS expressions, (ii) vitiation in the tissue dissemination and survival ability of the bacterium, and (iii) significant attenuation of the overall bacterial virulence. These results not only provide new insights into the regulation mechanisms of the Eth hemolysin and LuxS/AI-2 quorum sensing systems but also highlight the importance of these systems in bacterial virulence.
关键词	Edwardsiella Tarda Hemolysin Luxs Rnai Virulence
学科领域	Biotechnology & Applied Microbiology ; Microbiology
DOI	10.4014/jmb.0810.574
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000269364700004
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3036
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang Fang,Zhang, Min,Hu, Yong-hua,et al. Regulation of the Edwardsiella tarda Hemolysin Gene and luxS by EthR[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,2009,19(8):765-773.
APA	Wang Fang,Zhang, Min,Hu, Yong-hua,Zhang, Wei-wei,&Sun, Li.(2009).Regulation of the Edwardsiella tarda Hemolysin Gene and luxS by EthR.JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY,19(8),765-773.
MLA	Wang Fang,et al."Regulation of the Edwardsiella tarda Hemolysin Gene and luxS by EthR".JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 19.8(2009):765-773.

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