Construction of an attenuated Pseudomonas fluorescens strain and evaluation of its potential as a cross-protective vaccine

doi:10.1016/j.vaccine.2009.04.023

IOCAS-IR > 实验海洋生物学重点实验室

	Construction of an attenuated Pseudomonas fluorescens strain and evaluation of its potential as a cross-protective vaccine
	Wang, Huan-ran 1,2; Hu, Yong-hua 1,2; Zhang, Wei-wei 1,2; Sun, Li 1
	2009-06-19
发表期刊	VACCINE
ISSN	0264-410X
卷号	27 期号:30 页码:4047-4055
文章类型	Article
摘要	Ferric uptake regulator (Fur) is a global transcription regulator that is ubiquitous to Gram-negative bacteria and regulates diverse biological processes, including iron uptake, cellular metabolism, stress response, and production of virulence determinants. As a result, for many pathogenic bacteria, Fur plays a crucial role in the course of infection and disease development. In this study, the fur gene was cloned from a pathogenic Pseudomonas fluorescens strain, TSS, isolated from diseased Japanese flounder cultured in a local farm. TSS Fur can partially complement the defective phenotype of an Escherichia coli fur mutant. A TSS fur null mutant, TFM, was constructed. Compared to TSS, TFM exhibits reduced growth ability, aberrant production of outer membrane proteins, decreased resistance against host serum bactericidal activity, impaired ability to disseminate in host blood and tissues, and drastic attenuation in overall bacterial virulence in a Japanese flounder infection model. When used as a live vaccine administered via the injection, immersion, and oral routes, TFM affords high levels of protection upon Japanese flounder against not only P.fluorescens infection but also Aeromonas hydrophila infection. Furthermore, a plasmid, pJAQ, was constructed, which expresses the coding element of the Vibrio harveyi antigen AgaV-DegQ. TFM harboring pJAQ can secret AgaV-DegQ into the extracellular milieu. Vaccination of Japanese flounder with live TFM/pJAQ elicited strong immunoprotection against both V. harveyi and A. hydrophila infections. (C) 2009 Elsevier Ltd. All rights reserved.; Ferric uptake regulator (Fur) is a global transcription regulator that is ubiquitous to Gram-negative bacteria and regulates diverse biological processes, including iron uptake, cellular metabolism, stress response, and production of virulence determinants. As a result, for many pathogenic bacteria, Fur plays a crucial role in the course of infection and disease development. In this study, the fur gene was cloned from a pathogenic Pseudomonas fluorescens strain, TSS, isolated from diseased Japanese flounder cultured in a local farm. TSS Fur can partially complement the defective phenotype of an Escherichia coli fur mutant. A TSS fur null mutant, TFM, was constructed. Compared to TSS, TFM exhibits reduced growth ability, aberrant production of outer membrane proteins, decreased resistance against host serum bactericidal activity, impaired ability to disseminate in host blood and tissues, and drastic attenuation in overall bacterial virulence in a Japanese flounder infection model. When used as a live vaccine administered via the injection, immersion, and oral routes, TFM affords high levels of protection upon Japanese flounder against not only P.fluorescens infection but also Aeromonas hydrophila infection. Furthermore, a plasmid, pJAQ, was constructed, which expresses the coding element of the Vibrio harveyi antigen AgaV-DegQ. TFM harboring pJAQ can secret AgaV-DegQ into the extracellular milieu. Vaccination of Japanese flounder with live TFM/pJAQ elicited strong immunoprotection against both V. harveyi and A. hydrophila infections. (C) 2009 Elsevier Ltd. All rights reserved.
关键词	Aeromonas Hydrophila Cross Protection Ferric Uptake Regulator Live Attenuated Vaccine Pseudomonas Fluorescens
学科领域	Immunology ; Medicine, Research & Experimental
DOI	10.1016/j.vaccine.2009.04.023
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000267581500016
引用统计	被引频次：143[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3030
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Huan-ran,Hu, Yong-hua,Zhang, Wei-wei,et al. Construction of an attenuated Pseudomonas fluorescens strain and evaluation of its potential as a cross-protective vaccine[J]. VACCINE,2009,27(30):4047-4055.
APA	Wang, Huan-ran,Hu, Yong-hua,Zhang, Wei-wei,&Sun, Li.(2009).Construction of an attenuated Pseudomonas fluorescens strain and evaluation of its potential as a cross-protective vaccine.VACCINE,27(30),4047-4055.
MLA	Wang, Huan-ran,et al."Construction of an attenuated Pseudomonas fluorescens strain and evaluation of its potential as a cross-protective vaccine".VACCINE 27.30(2009):4047-4055.

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