Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties

doi:10.1016/j.dci.2008.10.001

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	Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties
	Sun, Baojian 1; Robertsen, Borre 1; Wang, Zhiqiang 2,3; Liu, Bin 2,3
	2009
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	33 期号:4 页码:547-558
文章类型	Article
摘要	A cluster of 11 interferon (IFN) genes were identified in the Atlantic salmon genome linked to the growth hormone I gene. The genes encode three different IFN subtypes; IFNa (two genes), IFNb (four genes) and IFNc (five genes), which show 22-32% amino acid sequence identity. Expression of the fish IFNs were studied in head kidney, leukocytes or To cells after stimulation with the dsRNA poly I:C or the imidazoquinoline S-27609. In mammals, poly I:C induces IFN-beta through the RIG-I/MDA5 or the TLR3 pathway, both of which are dependent on NF-kappa B. In contrast, S-27609 induces mammalian IFN-alpha in plasmacytoid dendritic cells through the TLR7 pathway independent of NF-kappa B. The presence of an NF-kappa B site in their promoters and their strong up-regulation by poly I:C, suggest that salmon IFNa1/IFNa2 are induced through similar pathways as IFN-beta. In contrast, the apparent lack of NF-kappa B motif in the promoter and the strong upregulation by S-27609 in head kidney and leukocytes, suggest that IFNb genes are induced through a pathway similar to mammalian IFN-alpha. IFNc genes showed expression patterns different from both IFNa and IFNb. Taken together, salmon IFNa and IFNb are not orthologs of mammalian IFN-beta and IFN-alpha, respectively, but appear to utilize similar induction pathways. (C) 2008 Elsevier Ltd. All rights reserved.; A cluster of 11 interferon (IFN) genes were identified in the Atlantic salmon genome linked to the growth hormone I gene. The genes encode three different IFN subtypes; IFNa (two genes), IFNb (four genes) and IFNc (five genes), which show 22-32% amino acid sequence identity. Expression of the fish IFNs were studied in head kidney, leukocytes or To cells after stimulation with the dsRNA poly I:C or the imidazoquinoline S-27609. In mammals, poly I:C induces IFN-beta through the RIG-I/MDA5 or the TLR3 pathway, both of which are dependent on NF-kappa B. In contrast, S-27609 induces mammalian IFN-alpha in plasmacytoid dendritic cells through the TLR7 pathway independent of NF-kappa B. The presence of an NF-kappa B site in their promoters and their strong up-regulation by poly I:C, suggest that salmon IFNa1/IFNa2 are induced through similar pathways as IFN-beta. In contrast, the apparent lack of NF-kappa B motif in the promoter and the strong upregulation by S-27609 in head kidney and leukocytes, suggest that IFNb genes are induced through a pathway similar to mammalian IFN-alpha. IFNc genes showed expression patterns different from both IFNa and IFNb. Taken together, salmon IFNa and IFNb are not orthologs of mammalian IFN-beta and IFN-alpha, respectively, but appear to utilize similar induction pathways. (C) 2008 Elsevier Ltd. All rights reserved.
关键词	Interferon Promoter Antiviral Fish Reptile Anolis Dsrna Virus Evolution
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2008.10.001
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000263781100016
引用统计	被引频次：126[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3016
专题	实验海洋生物学重点实验室
作者单位	1.Univ Tromso, Norwegian Coll Fishery Sci, Dept Marine Biotechnol, N-9037 Tromso, Norway 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.Chinese Acad Sci, Beijing Inst Genom, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Sun, Baojian,Robertsen, Borre,Wang, Zhiqiang,et al. Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2009,33(4):547-558.
APA	Sun, Baojian,Robertsen, Borre,Wang, Zhiqiang,&Liu, Bin.(2009).Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,33(4),547-558.
MLA	Sun, Baojian,et al."Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 33.4(2009):547-558.

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