Genetic mechanisms of multi-antimicrobial resistance in a pathogenic Edwardsiella tarda strain

doi:10.1016/j.aquaculture.2008.12.021

IOCAS-IR > 实验海洋生物学重点实验室

	Genetic mechanisms of multi-antimicrobial resistance in a pathogenic Edwardsiella tarda strain
	Sun, Kun 1,2; Wang, Hua-lei 3; Zhang, Min 1,2; Mao, Zhi-zhong 1; Sun, Li 1
	2009-04-03
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	289 期号:1-2 页码:134-139
文章类型	Article
摘要	TX01, a pathogenic Edwardsiella tarda strain isolated from diseased fish at an epidemic-inflicted fish farm in China, exhibits resistance to multiple classes of antimicrobial agents. The genes (kn(R). catA3, and tet(A), respectively) encoding resistance to kanamycin, chloramphenicol, and tetracycline were cloned and found to be 99-100% identical to the corresponding genes carried by known plasmids and transposons of human, animal, and environmental isolates. Further study demonstrated that TX01 harbors a plasmid, pETX, which proved to be (i) the carrier of the tet and cut operons; (ii) a mobile genetic element that is capable of transferring between bacteria of different genera. These results, which, to our knowledge, documented for the first time the co-existence of chloramphenicol and tetracycline resistance determinants on a conjugative plasmid in a pathogenic E tarda strain, indicated that gene acquisition via horizontal transferring of pETX-like mobile genetic entities may have played an important part in the dissemination of antimicrobial resistance and that there have existed for some time widespread genetic exchanges between bacteria of human, animal/fish, and environmental origins. (C) 2008 Elsevier B.V. All rights reserved.; TX01, a pathogenic Edwardsiella tarda strain isolated from diseased fish at an epidemic-inflicted fish farm in China, exhibits resistance to multiple classes of antimicrobial agents. The genes (kn(R). catA3, and tet(A), respectively) encoding resistance to kanamycin, chloramphenicol, and tetracycline were cloned and found to be 99-100% identical to the corresponding genes carried by known plasmids and transposons of human, animal, and environmental isolates. Further study demonstrated that TX01 harbors a plasmid, pETX, which proved to be (i) the carrier of the tet and cut operons; (ii) a mobile genetic element that is capable of transferring between bacteria of different genera. These results, which, to our knowledge, documented for the first time the co-existence of chloramphenicol and tetracycline resistance determinants on a conjugative plasmid in a pathogenic E tarda strain, indicated that gene acquisition via horizontal transferring of pETX-like mobile genetic entities may have played an important part in the dissemination of antimicrobial resistance and that there have existed for some time widespread genetic exchanges between bacteria of human, animal/fish, and environmental origins. (C) 2008 Elsevier B.V. All rights reserved.
关键词	Antimicrobial Resistance Genetic Exchange Gene Transfer Mobile Genetic Element Edwardsiella Tarda
学科领域	Fisheries ; Marine & Freshwater Biology
DOI	10.1016/j.aquaculture.2008.12.021
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000264942400021
引用统计	被引频次：67[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3014
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Hang Zhou EnzySource Biotechnol Co Ltd, Hangzhou, Zhejiang, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun, Kun,Wang, Hua-lei,Zhang, Min,et al. Genetic mechanisms of multi-antimicrobial resistance in a pathogenic Edwardsiella tarda strain[J]. AQUACULTURE,2009,289(1-2):134-139.
APA	Sun, Kun,Wang, Hua-lei,Zhang, Min,Mao, Zhi-zhong,&Sun, Li.(2009).Genetic mechanisms of multi-antimicrobial resistance in a pathogenic Edwardsiella tarda strain.AQUACULTURE,289(1-2),134-139.
MLA	Sun, Kun,et al."Genetic mechanisms of multi-antimicrobial resistance in a pathogenic Edwardsiella tarda strain".AQUACULTURE 289.1-2(2009):134-139.

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