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Cloning and expression of glucose regulated protein 78 (GRP78) in Fenneropenaeus chinensis
Luan, Wei1,2; Li, Fuhua1; Zhang, Jiquan1; Wang, Bing1; Xiang, Jianhai1
2009-02-01
Source PublicationMOLECULAR BIOLOGY REPORTS
ISSN0301-4851
Volume36Issue:2Pages:289-298
SubtypeArticle
AbstractGRP78 (78 kDa glucose-regulated protein), also known as BiP (immunoglobulin heavy-chain-binding protein), is an essential regulator of endoplasmic reticulum (ER) homeostasis because of its multiple functions in protein folding, ER calcium binding, and controlling of the activation of transmembrane ER stress sensors. In this report, we cloned the full length cDNA of GRP78 (FcGRP78) from Chinese shrimp Fenneropenaeus chinensis. This cDNA revealed a 2,325 bp with 1,968 bp open reading frame encoding 655 amino acids. This is the first reported GRP78 gene in Crustacea. The deduced amino acid sequence of FcGRP78 shared high identity with previously reported insect GRP78s: 86, 87 and 85% identity with GRP78s of Drosophila melanogaster, Aedes aegypti and Bombyx mori, respectively. Northern blot analysis shows that FcGRP78 is ubiquitously expressed in tissues of shrimp. Heat shock at 35A degrees C significantly enhanced the expression of FcGRP78 at the first hour, reached the maximum at 4 h post heat shock, dropped after that and resumed to the normal level until 48 h of post recovery at 25A degrees C. Additionally, differential expression of FcGRP78 was detected in haemocytes, hepatopancreas and lymphoid organ when shrimp were challenged by white spot syndrome virus (WSSV). We inferred that FcGRP78 may play important roles in chaperoning, protein folding and immune function of shrimp.; GRP78 (78 kDa glucose-regulated protein), also known as BiP (immunoglobulin heavy-chain-binding protein), is an essential regulator of endoplasmic reticulum (ER) homeostasis because of its multiple functions in protein folding, ER calcium binding, and controlling of the activation of transmembrane ER stress sensors. In this report, we cloned the full length cDNA of GRP78 (FcGRP78) from Chinese shrimp Fenneropenaeus chinensis. This cDNA revealed a 2,325 bp with 1,968 bp open reading frame encoding 655 amino acids. This is the first reported GRP78 gene in Crustacea. The deduced amino acid sequence of FcGRP78 shared high identity with previously reported insect GRP78s: 86, 87 and 85% identity with GRP78s of Drosophila melanogaster, Aedes aegypti and Bombyx mori, respectively. Northern blot analysis shows that FcGRP78 is ubiquitously expressed in tissues of shrimp. Heat shock at 35A degrees C significantly enhanced the expression of FcGRP78 at the first hour, reached the maximum at 4 h post heat shock, dropped after that and resumed to the normal level until 48 h of post recovery at 25A degrees C. Additionally, differential expression of FcGRP78 was detected in haemocytes, hepatopancreas and lymphoid organ when shrimp were challenged by white spot syndrome virus (WSSV). We inferred that FcGRP78 may play important roles in chaperoning, protein folding and immune function of shrimp.
KeywordGlucose-regulated Protein 78 Fenneropenaeus Chinensis Heat Shock Wssv Challenge
Subject AreaBiochemistry & Molecular Biology
DOI10.1007/s11033-007-9178-z
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Indexed BySCI
Language英语
WOS IDWOS:000262088300010
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Cited Times:39[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/2984
Collection实验海洋生物学重点实验室
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Luan, Wei,Li, Fuhua,Zhang, Jiquan,et al. Cloning and expression of glucose regulated protein 78 (GRP78) in Fenneropenaeus chinensis[J]. MOLECULAR BIOLOGY REPORTS,2009,36(2):289-298.
APA Luan, Wei,Li, Fuhua,Zhang, Jiquan,Wang, Bing,&Xiang, Jianhai.(2009).Cloning and expression of glucose regulated protein 78 (GRP78) in Fenneropenaeus chinensis.MOLECULAR BIOLOGY REPORTS,36(2),289-298.
MLA Luan, Wei,et al."Cloning and expression of glucose regulated protein 78 (GRP78) in Fenneropenaeus chinensis".MOLECULAR BIOLOGY REPORTS 36.2(2009):289-298.
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