Enrichment of the hydrogen-producing microbial community from marine intertidal sludge by different pretreatment methods

doi:10.1016/j.ijhydene.2009.10.025

IOCAS-IR > 实验海洋生物学重点实验室

	Enrichment of the hydrogen-producing microbial community from marine intertidal sludge by different pretreatment methods
	Liu, Hongyan 1,2,3; Wang, Guangce 1,2; Zhu, Daling 2; Pan, Guanghua 2
	2009-12-01
发表期刊	INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
ISSN	0360-3199
卷号	34 期号:24 页码:9696-9701
文章类型	Article
摘要	To determine the effects of pretreatment on hydrogen production and the hydrogen-producing microbial community, we treated the sludge from the intertidal zone of a bathing beach in Tianjin with four different pretreatment methods, including acid treatment, heat-shock, base treatment as well as freezing and thawing. The results showed that acid pretreatment significantly promoted the hydrogen production by sludge and provided the highest efficiency of hydrogen production among the four methods. The efficiency of the hydrogen production of the acid-pretreated sludge was 0.86 +/- 0.07 mol H(2)/mol glucose (mean +/- S.E.), whereas that of the sludge treated with heat-shock, freezing and thawing, base method and control was 0.41 +/- 0.03 mol H(2)/mol glucose, 0.17 +/- 0.01 mol H(2)/mol glucose, 0.11 +/- 0.01 mol H(2)/mol glucose and 0.20 +/- 0.04 mol H(2)/mol glucose, respectively. The result of denaturing gradient gel electrophoresis (DGGE) showed that pretreatment methods altered the composition of the microbial community that accounts for hydrogen production. Acid and heat pretreatments were favorable to enrich the dominant hydrogen-producing bacterium, i.e. Clostridium sp., Enterococcus sp. and Bacillus sp., However, besides hydrogen-producing bacteria, much non-hydrogen-producing Lactobacillus sp. was also found in the sludge pretreated with base, freezing and thawing methods. Therefore, based on our results, we concluded that, among the four pretreatment methods using acid, heat-shock, base or freezing and thawing, acid pretreatment was the most effective method for promoting hydrogen production of microbial community. (C) 2009 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.; To determine the effects of pretreatment on hydrogen production and the hydrogen-producing microbial community, we treated the sludge from the intertidal zone of a bathing beach in Tianjin with four different pretreatment methods, including acid treatment, heat-shock, base treatment as well as freezing and thawing. The results showed that acid pretreatment significantly promoted the hydrogen production by sludge and provided the highest efficiency of hydrogen production among the four methods. The efficiency of the hydrogen production of the acid-pretreated sludge was 0.86 +/- 0.07 mol H-2/mol glucose (mean +/- S.E.), whereas that of the sludge treated with heat-shock, freezing and thawing, base method and control was 0.41 +/- 0.03 mol H-2/mol glucose, 0.17 +/- 0.01 mol H-2/mol glucose, 0.11 +/- 0.01 mol H-2/mol glucose and 0.20 +/- 0.04 mol H-2/mol glucose, respectively. The result of denaturing gradient gel electrophoresis (DGGE) showed that pretreatment methods altered the composition of the microbial community that accounts for hydrogen production. Acid and heat pretreatments were favorable to enrich the dominant hydrogen-producing bacterium, i.e. Clostridium sp., Enterococcus sp. and Bacillus sp., However, besides hydrogen-producing bacteria, much non-hydrogen-producing Lactobacillus sp. was also found in the sludge pretreated with base, freezing and thawing methods. Therefore, based on our results, we concluded that, among the four pretreatment methods using acid, heat-shock, base or freezing and thawing, acid pretreatment was the most effective method for promoting hydrogen production of microbial community. (C) 2009 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.
关键词	Pretreatment Hydrogen Production Denaturing Gradient Gel Electrophoresis (Dgge) Microbial Community
学科领域	Chemistry, Physical ; Energy & Fuels ; Environmental Sciences ; Physics, Atomic, Molecular & Chemical
DOI	10.1016/j.ijhydene.2009.10.025
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000272856400007
引用统计	被引频次：62[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2974
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Liu, Hongyan,Wang, Guangce,Zhu, Daling,et al. Enrichment of the hydrogen-producing microbial community from marine intertidal sludge by different pretreatment methods[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2009,34(24):9696-9701.
APA	Liu, Hongyan,Wang, Guangce,Zhu, Daling,&Pan, Guanghua.(2009).Enrichment of the hydrogen-producing microbial community from marine intertidal sludge by different pretreatment methods.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,34(24),9696-9701.
MLA	Liu, Hongyan,et al."Enrichment of the hydrogen-producing microbial community from marine intertidal sludge by different pretreatment methods".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 34.24(2009):9696-9701.

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