Construction and evaluation of DNA vaccines encoding Edwardsiella tarda antigens

doi:10.1016/j.vaccine.2009.06.071

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	Construction and evaluation of DNA vaccines encoding Edwardsiella tarda antigens
	Jiao, Xu-dong 1,2; Zhang, Min 1; Hu, Yong-hua 1; Sun, Li 1
	2009-08-20
发表期刊	VACCINE
ISSN	0264-410X
卷号	27 期号:38 页码:5195-5202
文章类型	Article
摘要	Edwardsiella tarda is an opportunistic pathogen that can infect humans, animal, and fish. Two E. tarda antigens, Eta6 and FliC, which are homologues to an ecotin precursor and the FliC flagellin, respectively, were identified by in vivo-induced antigen technology from a pathogenic E. tarda strain isolated from diseased fish. When used as a subunit vaccine, purified recombinant Eta6 was moderately protective against lethal challenge of E. tarda in a Japanese flounder model, whereas purified recombinant FliC showed no apparent immunciprotectivity. Similarly, DNA vaccines based on eta6 and fliC in the form of plasmids pEta6 and pFliC induced, respectively, moderate and marginal protection against E. tarda infection. To improve the vaccine efficacy of eta6, a chimeric DNA vaccine, pCE6, was constructed, which encodes Eta6 fused in-frame to FliC. pCE6 was found to induce significantly higher level of protection than pEta6. Likewise, another chimeric DNA vaccine, pCE18, which expresses FliC fused to a previously identified E. tarda antigen Et18, elicited significantly stronger protective immunity than the DNA vaccine based on et18 alone. Fish immunized with pEta6 and pCE6 produced specific serum antibodies and exhibited significantly enhanced expression of the genes encoding elements that are involved in both innate and adaptive immune responses. Furthermore, the induction magnitudes of most of these genes were significantly higher in pCE6-vaccinated fish than in pEta6-vaccinated fish. (C) 2009 Elsevier Ltd. All rights reserved.; Edwardsiella tarda is an opportunistic pathogen that can infect humans, animal, and fish. Two E. tarda antigens, Eta6 and FliC, which are homologues to an ecotin precursor and the FliC flagellin, respectively, were identified by in vivo-induced antigen technology from a pathogenic E. tarda strain isolated from diseased fish. When used as a subunit vaccine, purified recombinant Eta6 was moderately protective against lethal challenge of E. tarda in a Japanese flounder model, whereas purified recombinant FliC showed no apparent immunciprotectivity. Similarly, DNA vaccines based on eta6 and fliC in the form of plasmids pEta6 and pFliC induced, respectively, moderate and marginal protection against E. tarda infection. To improve the vaccine efficacy of eta6, a chimeric DNA vaccine, pCE6, was constructed, which encodes Eta6 fused in-frame to FliC. pCE6 was found to induce significantly higher level of protection than pEta6. Likewise, another chimeric DNA vaccine, pCE18, which expresses FliC fused to a previously identified E. tarda antigen Et18, elicited significantly stronger protective immunity than the DNA vaccine based on et18 alone. Fish immunized with pEta6 and pCE6 produced specific serum antibodies and exhibited significantly enhanced expression of the genes encoding elements that are involved in both innate and adaptive immune responses. Furthermore, the induction magnitudes of most of these genes were significantly higher in pCE6-vaccinated fish than in pEta6-vaccinated fish. (C) 2009 Elsevier Ltd. All rights reserved.
关键词	Adjuvant Dna Vaccine Edwardsiella Tarda Flagellin Subunit Vaccine
学科领域	Immunology ; Medicine, Research & Experimental
DOI	10.1016/j.vaccine.2009.06.071
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000269071900007
引用统计	被引频次：122[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2928
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Jiao, Xu-dong,Zhang, Min,Hu, Yong-hua,et al. Construction and evaluation of DNA vaccines encoding Edwardsiella tarda antigens[J]. VACCINE,2009,27(38):5195-5202.
APA	Jiao, Xu-dong,Zhang, Min,Hu, Yong-hua,&Sun, Li.(2009).Construction and evaluation of DNA vaccines encoding Edwardsiella tarda antigens.VACCINE,27(38),5195-5202.
MLA	Jiao, Xu-dong,et al."Construction and evaluation of DNA vaccines encoding Edwardsiella tarda antigens".VACCINE 27.38(2009):5195-5202.

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