Identification and characterization of the AcrR/AcrAB system of a pathogenic Edwardsiella tarda strain

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	Identification and characterization of the AcrR/AcrAB system of a pathogenic Edwardsiella tarda strain
	Hou, Jin-hui 1,2,3; Hu, Yong-hua 1,2,3; Zhang, Min 1,2,3; Sun, Li 1
	2009-06-01
发表期刊	JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
ISSN	0022-1260
卷号	55 期号:3 页码:191-199
文章类型	Article
摘要	Edwardsielia tarda is one of the leading marine pathogens that can infect a wide range of cultured marine species. In this study, the acrR-acrAB cluster was cloned from TX1, a pathogenic E. tarda strain isolated from diseased fish. AcrR and AcrAB were found to be involved in resistance against acriflavine and methyl viologen, which positively regulate the expression of acrAB. AcrR negatively regulates its own expression and the expression of the acrAB operon, most likely by interacting with a 24-bp operator site that overlaps the putative promoter of acrA (PacrA). The repressive effect of AcrR on PacrA could be relieved by acriflavine, methyl viologen, and ethidium bromide, the presence of each of which enhanced transcription from PacrA. Interruption of the regulated expression of acrR by introducing into TX1 a plasmid that overexpresses acrR affected growth under stress conditions, AI-2 production, and bacterial virulence. In addition, mutational analyses identified a constitutively active AcrR mutant (named N215), which exhibits full repressor activity but is impaired in its ability to interact with the inducer. Overexpression of N215 produced the same kind of but moderately stronger effect on TX1 compared to that produced by overexpression of the wild-type acrR.; Edwardsielia tarda is one of the leading marine pathogens that can infect a wide range of cultured marine species. In this study, the acrR-acrAB cluster was cloned from TX1, a pathogenic E. tarda strain isolated from diseased fish. AcrR and AcrAB were found to be involved in resistance against acriflavine and methyl viologen, which positively regulate the expression of acrAB. AcrR negatively regulates its own expression and the expression of the acrAB operon, most likely by interacting with a 24-bp operator site that overlaps the putative promoter of acrA (PacrA). The repressive effect of AcrR on PacrA could be relieved by acriflavine, methyl viologen, and ethidium bromide, the presence of each of which enhanced transcription from PacrA. Interruption of the regulated expression of acrR by introducing into TX1 a plasmid that overexpresses acrR affected growth under stress conditions, AI-2 production, and bacterial virulence. In addition, mutational analyses identified a constitutively active AcrR mutant (named N215), which exhibits full repressor activity but is impaired in its ability to interact with the inducer. Overexpression of N215 produced the same kind of but moderately stronger effect on TX1 compared to that produced by overexpression of the wild-type acrR.
关键词	Acrab Acriflavine Acrr Antimicrobial Resistance Methyl Viologen Virulence
学科领域	Biotechnology & Applied Microbiology ; Microbiology
收录类别	SCI
语种	英语
WOS记录号	WOS:000268828300003
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2916
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Xuzhou Inst Technol, Xuzhou, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Hou, Jin-hui,Hu, Yong-hua,Zhang, Min,et al. Identification and characterization of the AcrR/AcrAB system of a pathogenic Edwardsiella tarda strain[J]. JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY,2009,55(3):191-199.
APA	Hou, Jin-hui,Hu, Yong-hua,Zhang, Min,&Sun, Li.(2009).Identification and characterization of the AcrR/AcrAB system of a pathogenic Edwardsiella tarda strain.JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY,55(3),191-199.
MLA	Hou, Jin-hui,et al."Identification and characterization of the AcrR/AcrAB system of a pathogenic Edwardsiella tarda strain".JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY 55.3(2009):191-199.

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