Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum

doi:10.1007/s00343-009-9230-z

IOCAS-IR > 实验海洋生物学重点实验室

	Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum
	Han Yifan 2; Mo Zhaolan 1; Mao Yunxiang 2; Xiao Peng 2; Li Jie1 ; Hao Bin 2; Guo Dongsheng 2
	2009-08-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	27 期号:3 页码:519-526
文章类型	Article
摘要	Vibrio anguillarum is a common bacterial pathogen in fish. However, little is known about its pathogenic mechanism, in part, because the entire genome has not been completely sequenced. We constructed a fosmid library for V. anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage. We characterized the library by end-sequencing 50 randomly selected clones. This generated 93 sequences with a total length of 57 485 by covering 1.4% of the whole genome. Of these sequences, 58 (62.4%) were homologous to known genes, 30 (32.3%) were genes with hypothetical functions, and the remaining 5 (5.3%) were unknown genes. We demonstrated the utility of this library by PCR screening of 10 genes. This resulted in an average of 6.2 fosmid clones per screening. This fosmid library offers a new tool for gene screening and cloning of V. anguillarum, and for comparative genomic studies among Vibrio species.; Vibrio anguillarum is a common bacterial pathogen in fish. However, little is known about its pathogenic mechanism, in part, because the entire genome has not been completely sequenced. We constructed a fosmid library for V. anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage. We characterized the library by end-sequencing 50 randomly selected clones. This generated 93 sequences with a total length of 57 485 by covering 1.4% of the whole genome. Of these sequences, 58 (62.4%) were homologous to known genes, 30 (32.3%) were genes with hypothetical functions, and the remaining 5 (5.3%) were unknown genes. We demonstrated the utility of this library by PCR screening of 10 genes. This resulted in an average of 6.2 fosmid clones per screening. This fosmid library offers a new tool for gene screening and cloning of V. anguillarum, and for comparative genomic studies among Vibrio species.
关键词	End-sequencing Fosmid Library Pcr Screening Vibrio Anguillarum
学科领域	Limnology ; Oceanography
DOI	10.1007/s00343-009-9230-z
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000270742000013
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2914
专题	实验海洋生物学重点实验室海洋生物分类与系统演化实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ocean Univ China, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Han Yifan,Mo Zhaolan,Mao Yunxiang,et al. Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(3):519-526.
APA	Han Yifan.,Mo Zhaolan.,Mao Yunxiang.,Xiao Peng.,Li Jie.,...&Guo Dongsheng.(2009).Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(3),519-526.
MLA	Han Yifan,et al."Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.3(2009):519-526.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
han-Construction and（630KB）			限制开放	--	浏览