Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum | |
Han Yifan2; Mo Zhaolan1; Mao Yunxiang2; Xiao Peng2; Li Jie1; Hao Bin2; Guo Dongsheng2 | |
2009-08-01 | |
发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY |
ISSN | 0254-4059 |
卷号 | 27期号:3页码:519-526 |
文章类型 | Article |
摘要 | Vibrio anguillarum is a common bacterial pathogen in fish. However, little is known about its pathogenic mechanism, in part, because the entire genome has not been completely sequenced. We constructed a fosmid library for V. anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage. We characterized the library by end-sequencing 50 randomly selected clones. This generated 93 sequences with a total length of 57 485 by covering 1.4% of the whole genome. Of these sequences, 58 (62.4%) were homologous to known genes, 30 (32.3%) were genes with hypothetical functions, and the remaining 5 (5.3%) were unknown genes. We demonstrated the utility of this library by PCR screening of 10 genes. This resulted in an average of 6.2 fosmid clones per screening. This fosmid library offers a new tool for gene screening and cloning of V. anguillarum, and for comparative genomic studies among Vibrio species.; Vibrio anguillarum is a common bacterial pathogen in fish. However, little is known about its pathogenic mechanism, in part, because the entire genome has not been completely sequenced. We constructed a fosmid library for V. anguillarum containing 960 clones with an average insert size of 37.7 kb and 8.6-fold genome coverage. We characterized the library by end-sequencing 50 randomly selected clones. This generated 93 sequences with a total length of 57 485 by covering 1.4% of the whole genome. Of these sequences, 58 (62.4%) were homologous to known genes, 30 (32.3%) were genes with hypothetical functions, and the remaining 5 (5.3%) were unknown genes. We demonstrated the utility of this library by PCR screening of 10 genes. This resulted in an average of 6.2 fosmid clones per screening. This fosmid library offers a new tool for gene screening and cloning of V. anguillarum, and for comparative genomic studies among Vibrio species. |
关键词 | End-sequencing Fosmid Library Pcr Screening Vibrio Anguillarum |
学科领域 | Limnology ; Oceanography |
DOI | 10.1007/s00343-009-9230-z |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000270742000013 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/2914 |
专题 | 实验海洋生物学重点实验室 海洋生物分类与系统演化实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ocean Univ China, Qingdao 266003, Peoples R China |
推荐引用方式 GB/T 7714 | Han Yifan,Mo Zhaolan,Mao Yunxiang,et al. Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(3):519-526. |
APA | Han Yifan.,Mo Zhaolan.,Mao Yunxiang.,Xiao Peng.,Li Jie.,...&Guo Dongsheng.(2009).Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(3),519-526. |
MLA | Han Yifan,et al."Construction and characterization of a fosmid library for pathogenic bacterium Vibrio anguillarum".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.3(2009):519-526. |
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