Molecular cloning and characterisation of prophenoloxidase (ProPO) cDNA from Fenneropenaeus chinensis and its transcription injected by Vibrio anguillarum

doi:10.1007/s11033-008-9292-6

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	Molecular cloning and characterisation of prophenoloxidase (ProPO) cDNA from Fenneropenaeus chinensis and its transcription injected by Vibrio anguillarum
	Gao, Hongwei 1,2,3; Li, Fuhua 1; Dong, Bo 1; Zhang, Qingli 1; Xiang, Jianhai1
	2009-05-01
发表期刊	MOLECULAR BIOLOGY REPORTS
ISSN	0301-4851
卷号	36 期号:5 页码:1159-1166
文章类型	Article
摘要	The prophenoloxidase(ProPO) gene was cloned from haemocytes of Chinese shrimp Fenneropenaeus chinensis by Rapid Amplification Complementary DNA Ends (RACE) method. The full-length cDNA of prophenoloxidase gene consists of 3040 bp with a 2061 bp Open Reading Frame (ORF), encoding 686 amino acids. Phylogenetic analysis revealed that it belongs to insect-type invertebrate prophenoloxidase gene family. To understand ProPO reaction for pathogeny's challenge in shrimp, the expressions of ProPO in different tissues were studied by real-time PCR after challenged by Vibrio anguillarum. The results showed that the expression level of ProPO gene in haemocytes was highest among three studied tissues including haemocytes, lymphoid organ and hepatopancreas. The time-course change of ProPO mRNA levels in challenge experiment showed that ProPO mRNA transcripts had the biggest change extent in lymphoid organ.; The prophenoloxidase(ProPO) gene was cloned from haemocytes of Chinese shrimp Fenneropenaeus chinensis by Rapid Amplification Complementary DNA Ends (RACE) method. The full-length cDNA of prophenoloxidase gene consists of 3040 bp with a 2061 bp Open Reading Frame (ORF), encoding 686 amino acids. Phylogenetic analysis revealed that it belongs to insect-type invertebrate prophenoloxidase gene family. To understand ProPO reaction for pathogeny's challenge in shrimp, the expressions of ProPO in different tissues were studied by real-time PCR after challenged by Vibrio anguillarum. The results showed that the expression level of ProPO gene in haemocytes was highest among three studied tissues including haemocytes, lymphoid organ and hepatopancreas. The time-course change of ProPO mRNA levels in challenge experiment showed that ProPO mRNA transcripts had the biggest change extent in lymphoid organ.
关键词	Cloning Expression Prophenoloxidase Fenneropenaeus Chinensis
学科领域	Biochemistry & Molecular Biology
DOI	10.1007/s11033-008-9292-6
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000264851600041
引用统计	被引频次：47[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2910
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Shandong Entry Exit Inspect & Quarantine Bur Peop, Qingdao, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Gao, Hongwei,Li, Fuhua,Dong, Bo,et al. Molecular cloning and characterisation of prophenoloxidase (ProPO) cDNA from Fenneropenaeus chinensis and its transcription injected by Vibrio anguillarum[J]. MOLECULAR BIOLOGY REPORTS,2009,36(5):1159-1166.
APA	Gao, Hongwei,Li, Fuhua,Dong, Bo,Zhang, Qingli,&Xiang, Jianhai.(2009).Molecular cloning and characterisation of prophenoloxidase (ProPO) cDNA from Fenneropenaeus chinensis and its transcription injected by Vibrio anguillarum.MOLECULAR BIOLOGY REPORTS,36(5),1159-1166.
MLA	Gao, Hongwei,et al."Molecular cloning and characterisation of prophenoloxidase (ProPO) cDNA from Fenneropenaeus chinensis and its transcription injected by Vibrio anguillarum".MOLECULAR BIOLOGY REPORTS 36.5(2009):1159-1166.

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