Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor

doi:10.1007/s11240-009-9523-0

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	Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor
	Deng, Xiangyuan 1,2; Qin, Song 1; Zhang, Qun 1,2; Jiang, Peng 1; Cui, Yulin 1,2; Li, Xiaokun 3
	2009-06-01
发表期刊	PLANT CELL TISSUE AND ORGAN CULTURE
ISSN	0167-6857
卷号	97 期号:3 页码:253-261
文章类型	Article
摘要	A human acidic fibroblast growth factor gene, hafgf, was successfully transferred into Laminaria japonica (kelp) gametophytes via microprojectile bombardment using the biolistic PDS-1000/He gene gun. Following phosphinothricin screening, PCR detection and Southern blot analysis, transgenic L. japonica gametophytes were cultivated in an illuminated bubble-column bioreactor to optimize growth conditions. A maximal final dry cell density of 1,695 mg l(-1) was obtained in a batch culture having an initial dry cell density of 129.75 mg l(-1). This was achieved using an aeration rate of 1.08 l air min(-1) l(-1) culture in a medium containing 1.5 mM inorganic nitrate and 0.15 mM phosphate. In addition, the relationship between different nitrogen sources and growth of transgenic gametophytes indicated that both urea and sodium nitrate were effective nitrogen sources for cell growth, while ammonium ions inhibited growth of these gametophytes.; A human acidic fibroblast growth factor gene, hafgf, was successfully transferred into Laminaria japonica (kelp) gametophytes via microprojectile bombardment using the biolistic PDS-1000/He gene gun. Following phosphinothricin screening, PCR detection and Southern blot analysis, transgenic L. japonica gametophytes were cultivated in an illuminated bubble-column bioreactor to optimize growth conditions. A maximal final dry cell density of 1,695 mg l(-1) was obtained in a batch culture having an initial dry cell density of 129.75 mg l(-1). This was achieved using an aeration rate of 1.08 l air min(-1) l(-1) culture in a medium containing 1.5 mM inorganic nitrate and 0.15 mM phosphate. In addition, the relationship between different nitrogen sources and growth of transgenic gametophytes indicated that both urea and sodium nitrate were effective nitrogen sources for cell growth, while ammonium ions inhibited growth of these gametophytes.
关键词	Microprojectile Bombardment Bubble-column Bioreactor Laminaria Japonica Gametophytes Human Acidic Fibroblast Growth Factor
学科领域	Biotechnology & Applied Microbiology ; Plant Sciences
DOI	10.1007/s11240-009-9523-0
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000265942400004
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2896
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Jinan Univ, Biopharmaceut Res & Dev Ctr, Guangzhou 510632, Guangdong, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Deng, Xiangyuan,Qin, Song,Zhang, Qun,et al. Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2009,97(3):253-261.
APA	Deng, Xiangyuan,Qin, Song,Zhang, Qun,Jiang, Peng,Cui, Yulin,&Li, Xiaokun.(2009).Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor.PLANT CELL TISSUE AND ORGAN CULTURE,97(3),253-261.
MLA	Deng, Xiangyuan,et al."Microprojectile bombardment of Laminaria japonica gametophytes and rapid propagation of transgenic lines within a bubble-column bioreactor".PLANT CELL TISSUE AND ORGAN CULTURE 97.3(2009):253-261.

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