Comparison of photobioreactors and optimal light regime for rapid vegetative propagation of transgenic Undaria pinnatifida gametophytes

doi:10.1002/jctb.2206

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	Comparison of photobioreactors and optimal light regime for rapid vegetative propagation of transgenic Undaria pinnatifida gametophytes
	Deng, Xiangyuan 1,2; Qin, Song 1; Zhang, Yichen 1,2; Jiang, Peng 1
	2009-10-01
发表期刊	JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
ISSN	0268-2575
卷号	84 期号:10 页码:1486-1492
文章类型	Article
摘要	BACKGROUND: Previously, tachyplesin gene (tac) has been successfully transferred into Undaria pinnatifida gametophytes using the method of microprojectile bombardment transformation. The objectives of this study were to compare and evaluate the performance of bubble-column and airlift bioreactors to determine a preferred configuration of bioreactor for vegetative propagation of transgenic U. pinnatifida gametophytes, and to then investigate the influence of light on vegetative propagation of these gametophytes, including incident light intensity, photoperiod and light quality to resolve the problems of rapid vegetative propagation within the selected bioreactor.; BACKGROUND: Previously, tachyplesin gene (tac) has been successfully transferred into Undaria pinnatifida gametophytes using the method of microprojectile bombardment transformation. The objectives of this study were to compare and evaluate the performance of bubble-column and airlift bioreactors to determine a preferred configuration of bioreactor for vegetative propagation of transgenic U. pinnatifida gametophytes, and to then investigate the influence of light on vegetative propagation of these gametophytes, including incident light intensity, photoperiod and light quality to resolve the problems of rapid vegetative propagation within the selected bioreactor. RESULTS: Experimental results showed that final dry cell density in the airlift bioreactor was 12.7% higher than that in the bubble-column bioreactor under the optimal aeration rate of 1.2 L air min(-1) L-1 culture. And a maximum final dry cell density of 2830 mg L-1 was obtained within the airlift bioreactor using blue light at 40 mu mol m(-2) s(-1) with a light/dark cycle of 14/10 (h). Polymerase chain reaction (PCR) analysis indicated that genes (bar and tac) were not lost during rapid vegetative propagation within the airlift bioreactor. CONCLUSION: The airlift bioreactor was shown to be much more suitable for rapid vegetative propagation of transgenic U. pinnatifida gametophytes than the bubble-column bioreactor in the laboratory. The use of blue light allows improvement of vegetative propagation of transgenic U. pinnatifida gametophytes. (C) 2009 Society of Chemical Industry
关键词	Bubble-column Bioreactor Airlift Bioreactor Vegetative Propagation Undaria Pinnatifida Gametophytes Light Transgenic
学科领域	Biotechnology & Applied Microbiology ; Chemistry, Multidisciplinary ; Engineering, Chemical
DOI	10.1002/jctb.2206
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000270403800009
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2894
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Deng, Xiangyuan,Qin, Song,Zhang, Yichen,et al. Comparison of photobioreactors and optimal light regime for rapid vegetative propagation of transgenic Undaria pinnatifida gametophytes[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2009,84(10):1486-1492.
APA	Deng, Xiangyuan,Qin, Song,Zhang, Yichen,&Jiang, Peng.(2009).Comparison of photobioreactors and optimal light regime for rapid vegetative propagation of transgenic Undaria pinnatifida gametophytes.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,84(10),1486-1492.
MLA	Deng, Xiangyuan,et al."Comparison of photobioreactors and optimal light regime for rapid vegetative propagation of transgenic Undaria pinnatifida gametophytes".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 84.10(2009):1486-1492.

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