Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri

doi:10.1007/s11033-008-9335-z

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	Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri
	Cong, Ming 1,2; Ni, Duojiao 1,2; Song, Linsheng 1; Wang, Lingling 1; Zhao, Jianmin 1; Qiu, Limei 1; Li, Ling 1,2
	2009-07-01
发表期刊	MOLECULAR BIOLOGY REPORTS
ISSN	0301-4851
卷号	36 期号:6 页码:1451-1459
文章类型	Article
摘要	Peroxiredoxin V (PRX V) is known as an atypical 2-cysteine peroxiredoxin that protects the organisms against various oxidative stresses and functions in signal transduction. The cDNA of a PRX V gene (designated as CfPRX) was cloned from scallop Chlamys farreri. The full-length sequence of CfPRX cDNA was of 2,179 bp with a 564 bp open reading frame encoding a peptide of 187 amino acids. Sequence comparison showed that CfPRX shared higher identities with PRX Vs than that with other isoforms of PRX, indicating CfPRX was a member of the PRX V family. Fluorescent real-time quantitative PCR analysis revealed the presence of CfPRX transcripts in gill filaments, adductor muscle, heart, gonad, kidney and hemocytes, and the stimulation of Listonella anguillarum significantly (P < 0.01) enhanced the mRNA expression of CfPRX in hemocyte. These results indicated that CfPRX was a constitutive and inducible acute-phase protein which was involved in the immune resistance to L. anguillarum stimulation.; Peroxiredoxin V (PRX V) is known as an atypical 2-cysteine peroxiredoxin that protects the organisms against various oxidative stresses and functions in signal transduction. The cDNA of a PRX V gene (designated as CfPRX) was cloned from scallop Chlamys farreri. The full-length sequence of CfPRX cDNA was of 2,179 bp with a 564 bp open reading frame encoding a peptide of 187 amino acids. Sequence comparison showed that CfPRX shared higher identities with PRX Vs than that with other isoforms of PRX, indicating CfPRX was a member of the PRX V family. Fluorescent real-time quantitative PCR analysis revealed the presence of CfPRX transcripts in gill filaments, adductor muscle, heart, gonad, kidney and hemocytes, and the stimulation of Listonella anguillarum significantly (P < 0.01) enhanced the mRNA expression of CfPRX in hemocyte. These results indicated that CfPRX was a constitutive and inducible acute-phase protein which was involved in the immune resistance to L. anguillarum stimulation.
关键词	Chlamys Farreri Fluorescent Real-time Quantitative Pcr Immune Response Listonella Anguillarum Peroxiredoxin
学科领域	Biochemistry & Molecular Biology
DOI	10.1007/s11033-008-9335-z
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000266454500034
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2886
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Cong, Ming,Ni, Duojiao,Song, Linsheng,et al. Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri[J]. MOLECULAR BIOLOGY REPORTS,2009,36(6):1451-1459.
APA	Cong, Ming.,Ni, Duojiao.,Song, Linsheng.,Wang, Lingling.,Zhao, Jianmin.,...&Li, Ling.(2009).Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri.MOLECULAR BIOLOGY REPORTS,36(6),1451-1459.
MLA	Cong, Ming,et al."Molecular cloning, characterization and mRNA expression of peroxiredoxin in Zhikong scallop Chlamys farreri".MOLECULAR BIOLOGY REPORTS 36.6(2009):1451-1459.

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