Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Enrichment and hydrogen production by marine anaerobic hydrogen-producing microflora | |
Cai JinLing1,2; Wang GuangCe1,3; Li YanChuan4; Zhu DaLing3; Pan GuangHua3 | |
2009-08-01 | |
发表期刊 | CHINESE SCIENCE BULLETIN |
ISSN | 1001-6538 |
卷号 | 54期号:15页码:2656-2661 |
文章类型 | Article |
摘要 | Acid, alkali, heat-shock, KNO(3) and control pretreatment methods applied to anaerobic sludge were evaluated for their ability to selectively enrich the marine hydrogen-producing mixed microflora. Seawater culture medium was used as the substrate. The hydrogen yield of pretreated microflora was higher than that of the un-pretreated control (P < 0.05). Among the pretreatment methods studied, heat-shock pretreatment yielded the greatest hydrogen production, which was 14.6 times that of the control. When the effect of initial pH on hydrogen production of heat-shock pretreated samples was studied, hydrogen was produced over the entire pH range (pH 4-10). The hydrogen yield peaked at initial pH 8 (79 mL/g sucrose) and then steadily decreased as the initial pH increased. Sucrose consumption was high at neutral initial pH. During the process of hydrogen production, pH decreased gradually, which indicated that the acquired microflora consisted of acidogenic bacteria.; Acid, alkali, heat-shock, KNO3 and control pretreatment methods applied to anaerobic sludge were evaluated for their ability to selectively enrich the marine hydrogen-producing mixed microflora. Seawater culture medium was used as the substrate. The hydrogen yield of pretreated microflora was higher than that of the un-pretreated control (P < 0.05). Among the pretreatment methods studied, heat-shock pretreatment yielded the greatest hydrogen production, which was 14.6 times that of the control. When the effect of initial pH on hydrogen production of heat-shock pretreated samples was studied, hydrogen was produced over the entire pH range (pH 4-10). The hydrogen yield peaked at initial pH 8 (79 mL/g sucrose) and then steadily decreased as the initial pH increased. Sucrose consumption was high at neutral initial pH. During the process of hydrogen production, pH decreased gradually, which indicated that the acquired microflora consisted of acidogenic bacteria. |
关键词 | Biological Hydrogen Production Marine Anaerobic Hydrogen-producing Microflora Pretreatment Initial Ph |
学科领域 | Multidisciplinary Sciences |
DOI | 10.1007/s11434-009-0399-5 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000268571300014 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/2882 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Res Ctr Algal Bioenergy & Environm Effect, Key Lab Marine Resources & Chem Tianjin, Tianjin 300457, Peoples R China 4.Kunming Univ Sci & Technol, Biotechnol Res Ctr, Kunming 650224, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Cai JinLing,Wang GuangCe,Li YanChuan,et al. Enrichment and hydrogen production by marine anaerobic hydrogen-producing microflora[J]. CHINESE SCIENCE BULLETIN,2009,54(15):2656-2661. |
APA | Cai JinLing,Wang GuangCe,Li YanChuan,Zhu DaLing,&Pan GuangHua.(2009).Enrichment and hydrogen production by marine anaerobic hydrogen-producing microflora.CHINESE SCIENCE BULLETIN,54(15),2656-2661. |
MLA | Cai JinLing,et al."Enrichment and hydrogen production by marine anaerobic hydrogen-producing microflora".CHINESE SCIENCE BULLETIN 54.15(2009):2656-2661. |
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