Nitrogen isotope fractionation during nitrate, ammonium and urea uptake by marine diatoms and coccolithophores under various conditions of N availability

	Nitrogen isotope fractionation during nitrate, ammonium and urea uptake by marine diatoms and coccolithophores under various conditions of N availability
	Waser, NA ; Yin, KD ; Yu, ZM; Tada, K ; Harrison, PJ ; Turpin, DH ; Calvert, SE
	1998
发表期刊	MARINE ECOLOGY PROGRESS SERIES
ISSN	0171-8630
卷号	169 页码:29-41
文章类型	Article
摘要	Stable isotopes of N provide a new approach to the study of algal production in the ocean, yet knowledge of the isotope fractionation (epsilon) in various oceanic regimes is lacking. Here we report large and rapid changes in isotope composition (delta(15)N) of 2 coastal diatoms and 2 clones (open and coastal) of a coccolithophore grown in the simultaneous presence of nitrate, ammonium and urea under varying conditions of N availability (i.e. N-sufficiency and N-starvation followed by N-resupply) and hence different physiological states, During N-sufficiency, the delta(15)N of particulate organic N (PON) was well reproduced, using a model derived from Rayleigh distillation theory, with constant epsilon similar to that for growth on each individual N source. However, following N-resupply, the variations in delta(15)N(PON) could be well explained only in the case of the open ocean Emiliania huxleyi, with epsilon similar to N-sufficient conditions. It was concluded that the mechanism of isotope fractionation changed rapidly with N availability for the 3 coastal clones. However, in the case of E. huxleyi isolated from the Subarctic Pacific Ocean, no evidence of a change in mechanism was found, suggesting that perhaps open ocean species can quickly recover from N-depleted conditions.; Stable isotopes of N provide a new approach to the study of algal production in the ocean, yet knowledge of the isotope fractionation (epsilon) in various oceanic regimes is lacking. Here we report large and rapid changes in isotope composition (delta(15)N) of 2 coastal diatoms and 2 clones (open and coastal) of a coccolithophore grown in the simultaneous presence of nitrate, ammonium and urea under varying conditions of N availability (i.e. N-sufficiency and N-starvation followed by N-resupply) and hence different physiological states, During N-sufficiency, the delta(15)N of particulate organic N (PON) was well reproduced, using a model derived from Rayleigh distillation theory, with constant epsilon similar to that for growth on each individual N source. However, following N-resupply, the variations in delta(15)N(PON) could be well explained only in the case of the open ocean Emiliania huxleyi, with epsilon similar to N-sufficient conditions. It was concluded that the mechanism of isotope fractionation changed rapidly with N availability for the 3 coastal clones. However, in the case of E. huxleyi isolated from the Subarctic Pacific Ocean, no evidence of a change in mechanism was found, suggesting that perhaps open ocean species can quickly recover from N-depleted conditions.
关键词	Isotope Fractionation N-15/n-14 Nitrogen Uptake Diatoms Coccolithophores Nitrate Ammonium Urea
学科领域	Ecology ; Marine & Freshwater Biology ; Oceanography
收录类别	SCI
语种	英语
WOS记录号	WOS:000075589000003
引用统计	被引频次：98[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2860
专题	海洋生态与环境科学重点实验室
作者单位	1.Univ British Columbia, Dept Earth & Ocean sci, Vancouver, BC V6T 1Z4, Canada 2.Chinese Acad Sci, Inst Oceanol, Shandong 266071, Peoples R China 3.Kagawa Univ, Fac Agr, Dept Bioresource Sci, Miki, Kagawa 76107, Japan 4.Queens Univ, Dept Biol, Kingston, ON K7L 3N6, Canada
推荐引用方式 GB/T 7714	Waser, NA,Yin, KD,Yu, ZM,et al. Nitrogen isotope fractionation during nitrate, ammonium and urea uptake by marine diatoms and coccolithophores under various conditions of N availability[J]. MARINE ECOLOGY PROGRESS SERIES,1998,169:29-41.
APA	Waser, NA.,Yin, KD.,Yu, ZM.,Tada, K.,Harrison, PJ.,...&Calvert, SE.(1998).Nitrogen isotope fractionation during nitrate, ammonium and urea uptake by marine diatoms and coccolithophores under various conditions of N availability.MARINE ECOLOGY PROGRESS SERIES,169,29-41.
MLA	Waser, NA,et al."Nitrogen isotope fractionation during nitrate, ammonium and urea uptake by marine diatoms and coccolithophores under various conditions of N availability".MARINE ECOLOGY PROGRESS SERIES 169(1998):29-41.

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